| Colorectal cancer（CRC）is one of the most commonmalignant tumor in our country, with the improving of people’s livingstandard, changing of dietary structure and aging of population,themorbidity and mortality of colorectal cancer keep rising year by year,especially for colon cancer, the morbidity of colorectal cancer maintainsstably. when it was diagnosed, most CRC patients have belonged toadvanced stage, it was a serious problem what damaged people’s healthand life. Recently years, although the biology characteristics andmetastasis regularity of CRC had attained great achievements on the basisof molecular biology，but for some CRC still can’t be explicitly diagnosisbefore it happened to occult metastasis.SOX gen family represents a family of transcription factors whichhave the characteristics of HMG-box（high mobility group-box）, itmainly involves in sex determination and embryonic development. SOX2belongs to group B of SOX gen family, and it expresses in adult’s manynormal tissue, such as the brain, retina, tongue, esophagus, lung andstomach, and so on, which is the key factor for regulating embryonicdevelopment. SOX2expresses in embryonic pluripotent stem cell andplays an extremely important role in maintaining embryonic stem cellself-renewal and proliferation, developing embryonic development intoother organizations. Recently,some research heve shown that SOX2genemay be involved in the tumorigenesis, development and metastasis of avariety of solid tumors. Research heve reported that SOX2geneexpression was interfered in colon cancer cell line, which obviouslyblocked the phase transition of cancer cells from the S phase to the G2/M, this experiment suggest the SOX2gene interference can restrain cancercell proliferation; the level of SOX2expression in pancreatic ductaladenocarcinoma was increased gradually from intraductal neoplasia,carcinoma in situ to infiltrating ductal carcinoma; SOX2high expressioncorrelates with postoperation recurrence and metastasis for CRC. SO far,there is no research relevant the difference expression level of SOX2incolon cancer tissue between synchronous distant metastases group andwithout distant metastases group in domestic and foreign, this experimentchoose postoperative specimens from synchronous distant metastasis andno distant metastasis colon cancer patients, compare to the differenceexpression of SOX2in two groups colon cancer tissues, explored therelationship between SOX2and colon cancer with synchronous distantmetastasis.Objective：The present experiment explores the expression of SOX2gene in colon cancer pathological tissues and relationship between SOX2gene and the occurrence of synchronous distant metastases. Methods:Diagnosed clearly and had complete medical record of70colon cancerpatients’ postoperative specimens was collected from gastrointestinalsurgery of Sichuan province people’s hospital from January2011to June2013.All cases have not accept neoadjuvant chemoradiotherapy beforeoperation. Including35patents with synchronous distant metastases(liver,lung, bone, and so on) and the same period of confirmed35cases withoutdistant metastasis. Utilizing immunohistochemistry to detecte the level ofSOX2gene expression in colon cancer patients’ surgical specimensbetween distant metastases group and without distant metastases group.Result: There was no the expression of SOX2in the adjacent mucosa,and it can be positive in the colon cancer tissue. About this70cases ofcolon cancer specimens, the rate of SOX2gene high expression is31.4%. About histologic type, grade of differentiation, T stage, tumor size, tumorlocation, there was no difference between distant metastases group andwithout distant metastases group, the difference was no statisticallysignificant(P＞0.05). the expression level of SOX2correlated withhistologic type, grade of differentiation, TNM stage, the depth of tumorinvasion, lymph-node metastases, distant metastasis and the expression ofp53(P＜0.05)，and it had no significant relationship with patents’age, sex,tumor size, tumor location(P＞0.05).The rate of SOX2gen highexpression in the distant metastasis group was obviously higher than thewithout distant metastases group, the difference was statisticallysignificant（P＜0.05).Conclusion: SOX2is highly expressed in highinvasiveness colon cancer. SOX2correlates with synchronous distantspread in colon cancer. |
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