Study On The Mechanism Of Ibuprofen Suppressing Proliferation And Metastasis In Cancer Cell Lines

Purpose In recent years based on the research of the mechanism of tumorigenesis,people found that inflammation plays an important role in tumorigenesis. The starting point ofthe tumor is always associated with infection and chronic inflammation. And there are full ofinflammatory cells in tumor microenvironment, the inflammatory factors secreting byinflammatory cells may promote proliferation and migration of tumor. Therefore, byinhibiting the inflammatory response to antitumor is expected to bring a new dawn for tumortreatment. Through the research on the antitumor function of the ibuprofen has been foundthat ibuprofen can reduce the occurrence of many cancer, inhibit the growth of canceroustissue. However, the potentioal mechanism of ibuprofen in antitumor is seldom clarified, especially in lung cancer, breast cancer and stomach cancer. This study aims to clarify the roleof ibuprofen in inhibiting the proliferation and migration in non-small cell lung cancer cellline NCI-H460, breast cancer cell line SKBR3and gastric cancer cell line MG–803.Methods We mainly used MTT method to detect the proliferation inhibition of threecancer cell lines after ibuprofen treatment for24,48and72h. FCM (flow cytometry) wasused to test cell cycle arrest and apoptosis. The wound healing assay and Transwell were usedto study directional cell migration in vitro. Real-time PCR (polymerase chain reaction) andWestern blot analysis was used to detect the mRNA and protein expression of survivin andE-cadherin after ibuprofen treatment.Results Ibuprofen treatment decreased cell growth by inducing cell cycle arrest andapoptosis. Here we showed that when three kinds of cancer cell lines were treated withibuprofen for48h,2mM ibuprofen significantly inhibited cell proliferation and metastasis in adose-dependent manner. When three kinds of cancer cell lines were treated with2mMibuprofen for12h, ibuprofen significantly inhibited cell migration. Moreover, Real-time PCRand Western blot analysis showed that ibuprofen led to a reduction in the expression ofsurvivin and elevation in the expression of E-cadherin at the mRNA and protein levels.Further, we demonstrate that ibuprofen increased the phosphorylation of AKT.Conclusions Our findings provide new evidence that ibuprofen suppresses threekinds of cancer cell lines proliferation and metastasis by inhibiting survivin expressionand elevating E-cadherin expression.