The Effect Of Aspirin On Colon Cancer Liver Metastasis And The Research On The Mechanisms

Objective: Colon cancer is one of the most common malignant tumors andincidence in our country has also increased in recent years. The prognosis of coloncancer is closely related to the liver and distant metastasis, and the liver is the mostcommon organ of colon cancer metastasis,10%-25%of colon cancer patients werediagnosed with liver metastases. A number of studies showed that: people takingaspirin regularly have lower colorectal cancer incidence and mortality, and the distantmetastasis were inhibited. This study aimed to investigate the inhibitory effect ofaspirin on liver metastases of colorectal cancer in mice and study the underlyingmechanisms.Methods: C26colon cancer cells were injected into mouse spleen, which isaimed to establish colon cancer liver metastasis model combining with splenectomy.The mice successful modeled were randomly divided into two groups, the controlgroup and aspirin groups, each group with10mice. Four weeks later, survival rateand liver metastasis was observed in each group. To detected the effect of aspirin oncolon cancer cell metastasis and invasion, we did scratches and transwell experimentsusing C26cells in vitro. In addition, we also studied mechanisms by detecting theexpression of EMT-related genes using RT-PCR and Western blot experiments.Results: The results showed that, compared with the control group, aspirinsignificantly reduced the number of liver metastases of colon cancer and reduced theweight of liver with tumors. The pathological results showed that aspirin reducedtumor cell heteromorphism. Meanwhile, survival analysis showed that aspirin canimprove survival rate of mice with colon cancer liver metastases. On the other hand,the transwell and scratches experiments suggested that aspirin can weaken themigration and invasion of colon cancer cells. Furthermore, compared with the controlgroup, aspirin can inhibit the expression of vimentin and promote the expression ofE-cadherin, which suggested that aspirin could inhibit the occurrence of EMT.Conclusion: aspirin inhibits liver metastasis of colon cancer significantly.