A Study Of Xpression Of GOLPH3and Its Biological Characterisics Of Ovarian Cancer

BackgroundOvarian cancer (ovarian carcinoma, OC) is a common malignancy of the femalereproductive system. It’s difficult to find in early period. Epithelial ovarian cancer (EOC)is the most common type and is gynecological cancer mortality in the highest type. Currentconventional treatment of ovarian cancer is still surgery, chemotherapy, endocrine therapyand radiation measures, but there are problems with poor efficacy of these treatments. Withthe development of molecular biology, tumor molecular targeted therapy has become thefocus of attention of many scholars. RNA interference technology is gradually used tostudy the mechanism of action of the target gene and related genes. Golgi phosphoprotein3(GOLPH3) is a cancer gene discovered in recent years, there is overexpressed in avariety of solid tumors and is closely related to tumor clinical stage, tumor grade, invasionand metastasis. GOLPH3play a role in a variety of biological processes involved in thedevelopment of many cancers, and its overexpression resulted in multiple adverse clinicaloutcomes. While the role of GOLPH3in the occurrence and development of ovariancancer is not clear.PurposeThis aims to study the expression of GOLPH3in ovarian serous adenocarcinoma andits relationship with clinicopathological features, and use the gene interferencemechanisms to clarify the role of GOLPH3in the development process of ovarian cancer.Materials and methods1. Expression of GOLPH3in normal ovarian tissues, benign sreous ovarian tumorsand ovarian serous cystadenocarcinoma were quantified using real time RT-PCR andWestern blotting, respectively.2. Construction of expression plasmid (GOLPH3-shRNA) and successfullytransformed to get transiently transfected cell model.3. Use of Cell Counting Kit-8(CCK8), clone formation experiment and transwell assay detect transfected of SW626cells proliferation, migration and invasion, respectively.Results1. GOLPH3expression was higher in epithelial ovarian carcinoma than in benignsreous ovarian tumors at the mRNA and protein level.GOLPH3positive expression rate ishigher in ovarian sreous cystadenocarcinoma.2. GOLPH3positive expression rate is correlates with the pathologic differentiation(p=0.019), the FIGO stage (p=0.042), tumors histological grade (p=0.044) and lymphaticmetastasis (p=0.000), but unassociated with age(p=0.881).Moreover, poorly-differentiatedtumor tissues has higher value of the GOLPH3protein(p＜0.05).3. After48hours transfection of plasmid in cell interference experiments, transfectionefficiency SW626cells was approximately75%, mRNA expression of GOLPH3interference group and protein expression levels were significantly lower (p <0.01).4. Experimental tumor cell proliferation was inhibited (p<0.01), and this inhibition isindependent of time, and clonogenic capacity, metastasis and invasion of ovarian cancercells was also significantly reduced, the difference was statistically significant (p <0.01).ConclusionGOLPH3plays a major role in ovarian serous adenocarcinoma development.Andtargeting gene interference can reduce ovarian cancer cell lines SW626expresstion ofGOLPH3, which can inhibit tumor cell proliferation and tumorigenicity and significantlyreduce tumor cell invasion and migration. This experiment supplies a theoreticalfoundation for early diagnosis and treatment of ovarian cancer genes potential targets.