The Protective Effects Of Rg1Against Oxidative Damage Of Human Umbilical Cord Mesenchymal Stem Cells

ObjectiveObserving the influence of Rg1against peroxidation injury of human umbilical cordmesenchymal stem cells at the aspects of morphology, proliferation, senescence, cytokinesecretion, intracellular protein expression, to explore whether Rg1has protective effect onhuman umbilical cord mesenchymal stem cells or not, as well as to explore the extent ofdamage repair and the possible mechanism. So as to serve the stem cell therapy and findways for the function restorating of nerve damage or other patients related.MethodsThe human umbilical cord mesenchymal stem cells between5to6generation werechosen, with different concentrations of Rg1, its effect on cell proliferation was observedand optimal drug concentration was filtered. Cells were divided into6groups: negativecontrol group (cells at the concentration of3.3×105cells/ml inoculated into medium,which contained stem cell growth factor at the concentration of50ng/m L, fetal bovineserum at the concentration of10%, penicillin and streptomycin at the concentration of100U/ml), model group (under the negative control group conditions, when cells got thefusion rate of80%, hydrogen peroxide was added to the concentration of1.6μM),3groupwith Rg1(culturing under the model group conditions for30minutes, then each added Rg1to the concentration of0.5μM,4μM,32μM, and cultured for36hours), the positivecontrol group (culturing under the model group conditions for30minutes, then addedvitamin C to the concentration of220μM, and cultured for36hours). Then we observedcell proliferation, aging, the levels of TNF-α and MDA in culture supernatant, as so as thelevels of p-p38, p-JNK, superoxide dismutase, catalase, glutathione expression in cells ofeach group.Results Human umbilical cord mesenchymal stem cells of the32μM ginsenoside Rg1groupgot the highest proliferation rate. Compared with the model group, as the dose increases,proliferation rate of Rg1group was increased significantly, while aging was reducedsignificantly, the expression of TNF-α, p-JNK, MDA and p-p38was decreasedsignificantly, the expression of catalase, superoxide dismutase, glutathione was increasedsignificantly (P <0.05). These indicators of the positive control group got the figures justbetween the two group with4μM、32μM Rg1, and the difference was statisticallysignificant (P <0.05). Compared with the negative control group, proliferation rate of themodel group, ginsenoside Rg1group and positive control group was decreasedsignificantly, while aging was increased significantly, the expression of TNF-α, p-JNK,MDA and p-p38was increased significantly, the expression of catalase, superoxidedismutase, glutathione was decreased significantly (P <0.05).Conclusion32μM is the optimal concentration for ginsenoside Rg1in promoting human umbilicalcord mesenchymal stem cells proliferation. Rg1can promote proliferation, delay senility,reduce the activity of TNF-α, p-JNK protein, p-p38protein, MDA, increase the activityof catalase, superoxide dismutase, glutathione, so as to produce a protective role inpromoting repair the peroxidation injury of human umbilical cord mesenchymal stem cells.Furthermore, ginsenoside Rg1with the concentration of32μM can offer much betterprotective effect than vitamin C against peroxide damage of human umbilical cordmesenchymal stem cells. However, ginsenoside Rg1, vitamin C can not repair the damageof human umbilical cord mesenchymal stem cells caused by hydrogen completely.