| Objective: The study of this subject is to cultivate cytology as the basis, establish the model of tumor bearing mice, inhibitory test in vitro on H22 cells, evaluate the effect of Polygonum cuspidatum water decoction. In order to found an effective and simple anticancer scheme.Methods: The experimental detection of Polygonum cuspidatum water decoction on the effect of H22 cells.Including in vitro inhibition experiment, in vivo inhibition experiments. Decoction preparation in Polygonum cuspidatum,And after the success of H22 cell culture, In vitro experiment,The main use of the MTS method to detect the inhibitory effect of Polygonum cuspidatum water decoction on mouse hepatoma H22 cells.And has carried on the experiment in vivo, by measuring the tumor weight, inhibition rate was calculated.Results: The successful construction of liver H22 tumor bearing mice model, and through the animal experiment verified the good inhibitory rate. In vivo and in vitro experiments, the effect of Polygonum cuspidatum water decoction of H22 cell, Polygonum cuspidatum water decoction dose and the inhibition rate is positive correlation, the time and the inhibition rate is positive correlation.Conclusion: Polygonum Decoction showed a dose dependent and time dependent on mouse H22 cell. For preclinical research laid the foundation. |
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