Analyzed The Expression And Regulation Mechanism Of MicroRNA In Aortic Dissection Of Stanford A Type

Objective: Through case-control study of differentially expressed microRNA(miRNA) in aortic media, to analyse the mi RNA which is related to the development of aortic dissection, as well as its potential targets and mechanisms.Methods: Media of Stanford type A aortic dissection were harvested from the patients who were aortic dissection of Stanford type A and underwent surgery in Shenzhen Sun Yat-Sen Cardiovascular Hospital from 2013 September to 2014 February, including 5 samples from dissection aortic media(case group, group A), 5 samples from relatively normal media at aortic dissection border of the same patients(self control group, group B). Media of normal aorta were harvested from the patients who were diagnosed as Coronary heart disease, and underwent surgery, including 5 cases(control group, group C), and exclusion of other aortic related diseases. All the samples are from the patients who were Han nationality, male and from South of China. We analyzed the differentially expressed miRNAs of 3 group based on the technology of Agilent human miRNA chip, besides, analyzed the target genes and signaling pathway.Results: When group A/B compared with group C, The patient’s age, BMI, hypertension, smoking history, drinking history had no statistically significant difference. Group A and group B compared with group C, there were 89 differentially expressed miRNAs. Group A compared with group B, there are 13 differentially expressed miRNAs. There were 16 differentially expressed miRNAs, and 23 related target genes, such as PTEN, BCL2, RAS and MAPK1, involved in 11 signaling pathway, such as MAPK signaling pathway, Focal adhesion and Regulation of actin cytoskeleton.Conclusion: 1. Focal adhesion and MAPK signaling pathway may play an important role in the occurrence and development of aortic dissection. 2. Apoptosis may be closely related to pathogenesis and progression of aortic dissection. 3. Study on the differential expression and regulation mechanism of mi RNA in aortic media has a great significance to explore the pathogenesis and progression of aortic dissection.