Association Between Serum Levels Of S100B Protein And Anti-ganglioside M1 Auto-antibodies In Children With ASD

Objective: To study the relationship between serum S100 B protein and anti-GM1 antibodies in ASD, understanding whether there is a link with serum S100 B protein and autoimmune antibodies in the pathogenesis of ASD. The result can provide some clues to the pathogenesis and treatment of ASD in future.Methods: The concentrations of serum S100 B protein and anti-GM1 antibodies have been detected by enzyme-linked immunosorbent assay(ELISA) in 40 children with ASD and 40 healthy children. The children with ASD are classificated to the extent of the disease by Childhood Autism Rating Scale(CARS). The children with ASD have been diagnosed by Children’s Health experts, who came from Children’s Hospital of Hunan. There are 32 males and 8 females, aged from 2 to 5 years, and the normal group are 30 males and 10 females healthy children, aged from 2 to 5 years. The age and gender are consistent with the patient group.Result:( 1) The levels of S100B(195.43±41.62pg/ml) in ASD group are significantly higher than the control group protein levels(151.88±21.06pg/ml)(p<0.001); The anti-GM1 antibodies levels(126.33±28.63ng/ml) in ASD group are higher than the control group protein levels(72.39±33.40ng/ml)(p<0.001);According to ROC curve, the value of truncated S00 B protein is 160.27 pg / ml, and the anti-GM1 antibodies is 117.4ng / ml.(2) The ASD group have 33 cases(82.5%), which are positive of S100 B protein.The control group has 14 cases(35.0%), which are positive of S100 B protein(p=0.000). The ASD group has 32 cases(80.0%), which are positive of anti-GM1 antibodies. The control group have 7 cases(17.5%), which are positive of anti-GM1 antibodies(p= 0.000).(3) The mean of S100 B protein expression levels(224.92±47.64 pg/ml) in female patients is significantly higher than the male patients(188.06±37.23 pg/ml)(p=0.023). The mean of anti-GM1 antibodies expression levels(149.53 ± 9.00ng/ml) in female patients is significantly higher than the male patients(120.52±28.97ng/ml)(p=0.009).(4) Severe female patients(7 cases) accounted for 87.5% of all female patients, severe male patients(12 cases) accounted for 37.5% of all male patients, the morbidity of severe female patients is significantly higher than severe male patients(p=0.017).(5) The Pearson correlation coefficient of serum S100 B protein and anti-GM1 antibodies in patients group and the control group is r=0.644, p=0.000.Conclusion:(1) The serum levels of S100 B protein and anti-GM1 antibodies in patients are higher, and their levels have a correlation with the severity of ASD.(2) Both S100 B protein and anti-GM1 antibodies in serum are correlated in the pathogenesis of ASD.