| Background:In recent years, the cardiovascular disease prevention is more and more important, Framingham Heart Study and carotid atherosclerosis are powerful method of cardiovascular events prediction. Inflammation has been shown to be an important factor in the occurrence of cardiovascular disease, which can be mediated by a variety of inflammatory cells, inflammatory factors on vascular endothelial erosion. Interleukin 18(IL-18) as a kind of important inflammatory cytokines, has been shown to associated with many cardiovascular diseases.Oxidative stress is an important pathway of vascular endothelial function in inflammatory damage and peroxidation nitrite anion(ONOO-) is the role of NO and O2- generated material. It can not only reflect endothelial nitric oxide(no) concentration, and is mediated by oxidative stress. Vascular pseudo VWF(von Willebrand factor VWF) and vascular endothelial cells and its main function is reflects the extent of damage of vascular endothelial cells to assess vascular endothelial function. There is less research to explore the people at risk of cardiovascular disease with CAS population’s vascularendothelial function. Objective: detection of people at risk of cardiovascular disease and with CAS human vascular endothelial function and plasma level of IL-18 changes, to explore the VI relationship between IL-18 and endothelial function, and on vascular endothelial injury mechanism. Methods:In January November 2013 to 2014 in Changsha Central hospital stroke prevention and screening of the population, through the Framingham equation method, questionnaire investigation, medical history collection, physical examination, color Doppler ultrasound examination of carotid artery in patients with high cardiovascular risk were randomly selected 56 people, according to with CAS points for high-risk with CAS group(n = 28) and pure high risk group(n = 28). At the same time, randomly selected cardiovascular low-risk populations at age and sex matched as controls(n=28). Extraction of selected plasma, Use enzyme linked immunosorbent assay( ELISA) were detected of interleukin-18(IL-18) in plasma of the population selected, v WF and ONOO- parallel marking material 3-nitrotyrosine(3-Tyrosine nitration, NT) of concentration. In in vitro experiments, the separation of calf serum, cultured human umbilical vein endothelial cells(human umbilical vein endothelial cells, HUVEC), different concentrations of recombinant human interleukin-18(0umol/L, 5umol / 20 umol, 50 umol / L, 100 umol / L, 200 umol / l was) join in HUVEC intervention and cultured for 24 hours, collecting supernatant, the concentration of no in the supernatant of cultured cells in each group was measured by Gressis method. Result:1. Patients with high cardiovascular risk in plasma VWF concentration [(975.02 + 425.15)ng/L] and NT in plasma concentration [(60.46 + 22.85)ng/L] compare with the control group [(446.48 + 257.21)ng/L] and [(37.15 + 4.43)ng/L] were increased significantly, the difference was statistically significant(P < 0.05).2 In patients with cardiovascular risk, plasma v WF concentrations were positively correlated with plasma NT concentrations(P<0.05, r=0.587).3. In patients with high cardiovascular risk, risk with CAS group, the plasma VWF levels[(1058.50 + 370.68)ng/L] compare with pure high-risk group [(891.54 + 465.13)ng/L] were increased significantly, the difference was statistically significant(P < 0.05); high risk with CAS group, the concentration of NT in plasma [(55.32 + 20.20)ng/L] compare with pure high-risk group [(52.84 + 18.81)ng/L] were increased significantly,the difference was not statistically significant(P > 0.05).4. Patients with high cardiovascular risk of plasma IL-18 concentration [(39.93 + 13.35) ng/L]compare with control group [(18.10 + 10.99)ng/L] were significantly increased, the difference was statistically significant(P < 0.05).5. In patients with high cardiovascular risk, and was positively correlated with plasma IL-18 concentration and the concentration of NT in plasma(0.560, P < 0.05); in patients with high cardiovascular risk, the plasma IL-18 concentration and plasma VWF concentration was positively related(r = 0.893, P < 0.05).6.In patients with high cardiovascular risk in high-risk with CAS group, the plasma levels of IL-18 in [(39.93 + 5.18) ng/L]compare with pure high-risk group [(36.96 + 7.18) ng/L], the difference was not statistically significant,(P > 0.05).7. in vitro experiment, different concentrations of recombinant human IL-18(5umol / L, 20 umol, 50 umol / L, 100 umol / L, 200 umol / l was) intervention HUVEC supernatant concentration of NO respectively: [(663.88 + 95.74)umol/L], [(71.519 + 113.56)umol/L], [(791.84 + 118.64)umol/L], [(1061.02 + 67.58)umol/L],[(2460.06 + 338.26)umol/L] than 0umol/L concentration intervention control group [(577.34 + of 79.95)umol/L] were increased in comparison,(P < 0.05), and showed a concentration dependent increase. Conclusion :1 Cardiovascular risk patients with endothelial function has been injured.2.IL-18 mediates the damage of endothelial function in cardiovascular risk population.3.NO can promote the generation of ONOO- by increasing the production of IL-18, and can promote the occurrence of endothelial injury. |
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