The Based Metabonomics Analysis And The Effects Of Different Fluid Resuscitation Strategy In Rats With Hemorrhagic Shock

Objective : To investigate the changes of plasma metabonomics and influences of different liquid resuscitation strategies on plasma indexes of plasma metabolism and intracranial dialysate in rats with hemorrhagic shock.Methods:(1)The male SD rats weighting 260- 280 g were selected to induce the rat model of hemorrhagic shock. According to the experimental design, the rats were assigned into five groups(n=10): Control group(C), No fluid resuscitation group(NF group), Lactate ringer solution group(LRS group), hydroxyethyl starch group(HES) and Lactate ringer solution plus hydroxyethyl starch group(LRS + HES group). The corresponding fluid resuscitation strategy was carried out 1h after the shock followed by exsanguination 2 h later. The blood sample and intracranial dialysate were collected at 2 h post-resuscitation. The levels of lactic acid, pyruvic acid, glucose and acetone in plasma and intracranial dialysate were measured among five groups. The plasma activities of aspartate aminotransferase(AST), creatine kinase(CK), alkaline phosphatase(ALB) and alanine aminotransferase(ALT) were measured among five groups.(2)Twenty male SD rats weighting 260- 280 g were randomly divided into Sham group and HS group(10 for each group). Only the femoral surgery without intubation was undergoing in Sham group, while the left femoral artery of HS group was intubated with a tracheal as to maintain the blood pressure at 40 mm Hg. Blood samples(2 ml) were collected after 90 min and the 1H-nuclear magnetic spectroscopy was employed to evaluate the metabolic profile of plasma. The changes of plasma metabolites were further analyzed by principal component analysis(PCA) and partial least-squares discriminant analysis(PLS-DA).Results:(1)Compared with C group, there were increased plasma levels of lactic acid and acetone but decrease levels of pyruvic acid and glucose in NF group with significant differences(P<0.05). There was no significant difference between LRS+HES group and C group. Besides the lactic acid in LRS group, the above four indicators of C, HES and LRS+HES groups were superior to those of NF group(P<0.05). Among those groups, the above four indicators of LRS+HES groups were superior to those of LRS and HES groups(P<0.05).Compared with C group, there were increased intracranial dialysate levels of lactic acid and acetone but decrease levels of pyruvic acid and glucose in NF group with significant differences(P<0.05). There was no significant difference between LRS+HES group and C group. Besides the lactic acid and glucose in LRS group, the above four indicators of C, HES and LRS+HES groups were superior to those of NF group(P<0.05). Among those groups, the above four indicators of LRS+HES groups were superior to those of LRS and HES groups(P<0.05).Compared with C group, the plasma activities of AST, CK, ALB and ALT had no significant difference among five groups(P>0.05).The 1H-NMR spectra significantly varied between both groups. As was shown in PCA analysis, the distributions of the integral value in both groups partly overlapped with a more obvious separation trend. The PLS-DA analysis showed a more concentrated distribution of both groups without overlap. The PCA and PLS-DA corresponding load chart showed that most products mainly centralized around the origin without significant difference. Only a few moved outward away from the origin. Compared with Sham group, the levels of fatty acid(δ2.20, 2.22~2.25), acetic acid(δ0.75), O-acetyl-glycoprotein(δ2.01~2.05), N-acetylglycoprotein(δ2.13~2.16) and VLDL/LDL(δ0. 82, 1.26~1.34) significantly decreased.Conclusion: The plasma 1H-NMR-based metabolomics in HS in rats significantly changed. There were disordered plasma metabolic indicators in rats with hemorrhagic shock. Timely fluid resuscitation can alleviate metabolic disorders in hemorrhagic shock with better effect in lactate ringer solution plus hydroxyethyl starch.