MRNA Expressions And Clinical Significances Of T-bet, GATA-3, ROR γt And Foxp3 In Peripheral Blood Of Patients With Chronic Lymphocytic Leukemia

Objective:The study was to investigate the mRNA expression of T-bet,GATA-3, RORyt,Foxp3mRNA in peripheral blood of patients with chronic lymphocytic leukemia (CLL) and explore their potential role in the pathogenesis and clinical diagnosis.Methods:A total of 46 newly diagnosed and untreated patients with CLL was chosen as a patient group, including 16 patients in the stage of Binet A,15 in the stage of Binet B,and 15 in the stage of Binet C;20 healthy persons were selected as controls.The quantitative fluorescence PCR was adopted to detect the mRNA expression of T-bet,GATA-3,RORyt and Foxp3 in peripheral blood mononuclear cell(PBMC). Application software SPSS 17.0 statistical was used for statistical analysis,quantitative data was sigified by the mean ± standard. T test was used as comparing between groups while ANOVA can be used among the three groups. P<0.05 prompted that difference was statistically significant.Results:(1).The expression of T-bet mRNA in patient group was lower than that in normal controls (P<0.05), while the mRNA expression of GATA-3 mRNA, ROR yt and Foxp3 in CLL patients group were higher than that in normal controls(P<0.05),and the ratio of T-bet/GATA-3 and RORyt/Foxp3 in CLL patients were lower than that in normal controls(P<0.05).(2).The later the stage, the higher the mRNAexpression of GATA-3 and Foxp3. The mRNA expression of GATA-3 mRNA in stage Binet B and stage Binet C of CLL patients were higher than that in stage Binet A (P<0.05),and the mRNA expression of Foxp3 in stage Binet C was higher than that in stage of Binet A and Binet B(P<0.05); the later the stage, the lower the ratio of T-bet/GATA-3 and RORyt/Foxp3. The ratio of T-bet/GATA-3 in stage of Binet A CLL patients was higher than that in stage Binet C (P<0.05) and the ratio of RORyt/Foxp3 in stage of Binet A and Binet B were higher than that in stage Binet C (P<0.05).Conclusions:(1).This study found in the level of transcription factors in CLL patients that the mRNA expressions of GATA-3 and Foxp3 were high and the balances of Th1/Th2 and Th17/Treg were broken. (2).with the process of disease, the balance shifts from Th1/Th2 and Th17/Treg to Th17 and Treg; (3). By detecting the GATA-3 mRNA and Foxp3 mRNA may contribute to assess prognosis and guide clinical treatment.