Patients With BDNF Gene Promoter Methylation CPG Correlation Stress And Severe Depression

Objective:To investigate the effect of stress on depression in patients with severe brain-derived neurotrophic factor (Brain-derived neurotrophic factor, BDNF) gene methylation in the pathogenesis of severe depression, and brain-derived neurotrophic factor (BDNF) gene methylation and the relationship between major depressive disorder, a clear genetic, neurological and environmental factors in the pathogenesis of the possible interaction between severe depressive disorder pathogenesis explore from severe depression level ofmolecular biology, and life events on the onset of severe depression possible ways to further explore the major depressive disorder etiology, pathogenesis, and barriers to early diagnosis, early intervention, individualized treatment for severe depression and to provide a theoretical basis for the prevention of disease, provide more information. Methods:A case-control study was conducted in strict accordance with DSM-IV diagnostic criteria, selected 48 cases of major depressive disorder, according to the Life Events Scale subdivided into stress group (LES≥20 points) and non-stress group and 27 cases with the sex, age-matched normal controls for the study. Using HAMD-17 assessment of the severity of depression. Subjects drawn peripheral venous whole blood, genomic DNA, by weight bisulfite modification, methylation status of direct sequencing of brain-derived neurotrophic factor (BDNF) in the promoter region CpG islands quantitative detection. SPSS17.0 using statistical package for data processing. Analysis of major depressive disorders stress group, non-stress group and normal control group of brain-derived neurotrophic factor (BDNF) in the promoter region methylation differences 19CPG sites and the overall level of methylation in the three groups; significance level test are used a=0.05. Results:A severe depressive disorder stress group, non-stress group Brain-derived neurotrophic factor (BDNF) promoter CPG1, CPG2, CPG3, CPG4, CPG5, CPG6, CPG7, CPG9, CPG11, CPG12, CPG13, CPG15, CPG16, CPG17, water CPG19 methylated sites were higher than the normal control group, the difference was statistically significant (P<(0.05 0.001) CPG10, CPG14, CPG18sites, no significant difference between P) three groups.>; severe comparison of major depressive disorder and major depressive disorders stress group of non-stressed group of brain-derived neurotrophic factor (BDNF) promoter methylation levels CPG sites, the difference was not statistically significant.2, severe depression stress group, a non-stress group and the normal control group of brain-derived neurotrophic factor (BDNF) promoter CPG start comparing the overall methylation level, the difference was statistically significant (P<0.001); severe depressive disorder comparison of the two groups of brain-derived neurotrophic factor (BDNF) promoter methylation of CPG overall level, the difference was not statistically significant (P>0.05). Conclusion:stress may be associated with major depressive disorder Brain-derived neurotrophic factor (BDNF) gene promoter CpG methylation no close relationship, stressful life events may not be major depressive disorder Brain-derived neurotrophic factor (BDNF) gene promoter CpG sequences direct cause of methylation; brain-derived neurotrophic factor (BDNF) gene promoter region methylation sites CPG and major depressive disorders are closely related, CpG methylation levels of brain-derived neurotrophic factor (BDNF) gene promoter region as severe depressive disorder diagnostic biomarkers; BDNF gene promoter methylation of CPG overall level closely with major depressive disorder, which may affect the incidence of severe depressive disorder.