Dopamine2 Receptor Take Part In The Inhibitory Effects Of Prenatal Alcohol Exposure To Rhythmic Respiratory Discharge Activities Of Neonatal Rat

BackgroundAlcoholism is a important factor that influence human health. It not only to the individual physical and psychological health damage, and have negative effects on society. Women alcohol consumption will seriously affect fetal growth and development of alcohol consumption during pregnancy, even fetal alcohol syndrome (fetal alcohol syndrome, FAS), and central nervous system disease is the most important consequence of all. Stable basic breathing rhythm originated in the medullary respiratory center breathing rhythm, and it is very important to maintain the body’s normal functions. Central respiratory diseases, respiratory diseases, in particular, is a common and frequent diseases in clinical work, central breathing more alcohol induced abnormal, therefore, we study the effect of prenatal alcohol on respiratory center.ObjectiveTo investigate the mechanism of prenatal alcohol exposure inhibition respiratory rhythm discharge activity of neonatal rat brainstem slice, and make it clear that whether dopamine 2 receptor (D2R) take part in this inhibition.MethodsSPF female SD rats were randomly divided into control group and experimental group. Both two groups been breed same rats except in the prenatal alcohol exposed rats 8%(V/ V) ethanol solution as the only drinking water, from mating and throughout pregnancy.1. Medullary slices from 2 days neonatal rats used to record RRDA. Observe the effects of D2R agonists Quinpirole to the control group and experimental group RRDA, and the effects of t Raclopride, make it clear that D2R in prenatal alcohol exposure on inhibition of the newborn rat medullary respiratory center.2. Western blot was using to detect the expression of D2R protein level in pre-BotC respiratory neurons of both group.3. qRT-PCR was using to detect the expression of D2R mRNA level in pre-BotC respiratory neurons of both group.Results1. The control group and the experimental group section discharge stability without attenuation in 60min, make the experimental model is stable and reliable. The experimental group than in the control group of slices. The D2R agonist Quinpirole inhibits slices in the two group, and control group is stronger than the excitatory effects of the experimental group. The D2R antagonist Raclopride two groups put the excitatory effect of section, while the control group is stronger than inhibition of the experimental group.2. Western Blot results show that prenatal alcohol exposure, express cut D2R protein level with the newborn rat bone marrow.3. Fluorescence quantitative PCR results showed that prenatal alcohol exposure, D2R mRNA to decrease the level of medullaly film in neonatal rats.Conclusions1. Medulla chip prenatal alcohol exposure suppress RRDA and regulating effects of D2R to RRDA.2. Prenatal alcohol exposure down-regulating the expression of D2R mRNA and its protein, this is a possible mechanism that prenatal alcohol exposure suppress RRDA of neonatal rat.