The Study Of Therapeutical Effect Of Ferulic Acid On Chronic Wound

Objective: To study whether ferulic acid can promote healing on chronic wounds in diabetic rats and rabbit ear chronic ischemic wounds and its possible mechanisms 。 Methods : Normal control group contains 20 male SD rats. high-glucose-high-fat combined with intraperitoneal injection of 45 mg / kg streptozotocin to form 80 diabetic rats. And they were randomly divided into four groups : the diabetic control group(DWC), the vaseline smear group(VWC), the 5% ferulic acid(5%FA) smear group and the 20 mg / kg ferulic acid intragastric administration groups(FA20). 2cm × 2cm full thickness dermal wounds was made on the backs of rats.The rats of the normal control group and the diabetic control group were only changed dressing in time, the others were changed wound dressing and given by intragastric administration daily. 40 male New Zealand White rabbits were randomly divided into 4 groups: vaseline group, ischemic control group, 5% ferulic acid group and Recombinant Bovine Basic Fibroblast Growth Factor For External Use(rb-bFGF) group.Rabbit ears ventral wound were made after the ischemic preconditioning and verification of ischemic effects. The ischemic control group were only changed dressing in time, and the others were changed wound dressing and given medicines daily. The two experiments had been observed. And we took photographs of the changing wound of all groups on the 1 、5、8、11、14、17、20 day and drawn specimens from them respectively. The wound healing rates were calculated at each time point and the wound healing time in each group were compared. Every specimen was divided into two parts since the center line.One was examined with HE staining to observe the wound healing and made for immumohistochemical staining to observe the expression of the VEGF、TGF-β1、CD31. The other’s RNA was extracted to detect the expression of the VEGF、the TGFβ1 and the HIF-1α by Real-time PCR. Results: Rats:The general observation、the comparison of the wound healing rates at each time point in each group、the comparison of the duration of wound healing in each group and the HE staining of each specimen 8 days after operation are all indicate that the 5 % FA group and the FA20 group have longer duration of wound healing than that of the DWC group and the VWC group, but shorter than NWC group. The result of the immunohistochemical staining indicates that the expression of VEGF and TGFβ1 were up-regulated in the 5%FA group and the FA20 group compared with the DWC group and the VWC group,but donw- regulated compared with the NWC group. The result of the PCR show that 5%FA group and FA20 group have more expression level of VEGF、TGFβ1、HIF-1α than that of the VWC group,but less than NWC group. Rabbits: The general observation、the comparison of the duration of wound healing in each group and the HE staining of each specimen 11 days after operation are all indicate that the duration of wound healing of the 5% ferulic acid group is similar to that of the bFGF group and markedly shorter than the ischemic control group and the vaseline smear group. The result of the immunohistochemical staining indicates that the content of the VEGF and CD31 of the 5 % ferulic acid groups and the bFGF group makes little difference, but there is markely less VEGF and CD31 in ischemic control group and the vaseline smear group. The result of the PCR shows that expression quantity of VEGF、TGFβ1、HIF-1α in the 5 % ferulic acid groups is similar to that in the bFGF group and the vaseline smear group but is obviously more than the ischemic control group and the vaseline smear group,P<0.05. Conclusions: Ferulic acid can promote angiogenesis by increasing VEGF, TGFβ1 and HIF-1α which are closely related to angiogenesis, and then promot the healing of chronic wounds.