Inhibition Effects Of Cisplatin-implants Against Human Gastric Cancer In Nude Mice

Objective: to observe the study cisplatin implants on gastric cancer nude mouse transplantation tumor growth and the spleen tyrosine kinase(striking tyrosine kinase, SYK) and human epidermal growth factor receptor 2(human epidermal growth factor receptor 2, HER2), adhesion molecule CD44 variants 6(CD44v6), lack of people on chromosome 10 protein phosphatase tension of homologous genes(phosphate and recogniton homology does on chromsome ten, PTEN), aspartic acid with cysteine protein hydrolase 3(cysteinyl aspartate specific protease, 3Caspase3), Survivin or apoptosis inhibiting gene expression level(Survivin) in gastric cancer tissues and the effect of exploration to discuss the effect of cisplatin implants inhibition of gastric cancer, and to clarify cisplatin slow-release implants in the occurrence and development of gastric cancer in the treatment of advantage.Methods: through of SGC7901 gastric cancer cell lines in BALB/c nude mice subcutaneous transplantation tumor model is set up,immediately after the success of the building is divided into were randomly divided into A, B, c, D group: group A, the PBS tail intravenous therapy, group B- by cisplatin tail intravenous therapy, group c- through injection of cisplatin tumor therapy, group D- by cisplatin slow-release implants implant therapy in tumor. Each group were observed after drug intervention tumors had the size and quality, and use a variety of detection method and contrast, differences in expression of the tumors had organization constituting the spleen tyrosine kinase(striking tyrosine kinase, SYK) and human epidermal growth factor receptor 2(human epidermal growth factor receptor 2, HER2), adhesion molecule CD44 variants 6(CD44v6), lack of people on chromosome 10 protein phosphatase and tension of the homologous genes(phosphate and recogniton homology does on chromsome ten, PTEN), aspartic acid with cysteine protein hydrolase 3(cysteinyl aspartate specific protease, 3Caspase3), Survivin or apoptosis inhibiting gene expression level(Survivin) in gastric cancer tissues.Results: the nude mice(20) in about ten days or so into the tumor,tumor rate is about 94%. The tumors had the volume is as follows: group A(1564.198 + 180.288) mm3, group B(883.334 + 81.749) mm3,583.776 + /- 73.946) mm3 group C and group D(295.859 + 146.474)mm3, group D with the rest of the three groups(A, B, C) after comparing the differences were statistically significant(P < 0.05). Groups the expression of SYK, respectively is: A(5.06 + 5.06) %,(14.14 + 2.84) %B, C(30.42 + 3.92) %, D(73.42 + 4.92) %, increasing expression and have statistical significance; The expression of HER2, respectively is: A(77.34 + 77.34) %,(45.80 + 6.60) % B, C(34.82 + 7.32) %, D(16.32 +4.82) %, diminishing expression and have statistical significance; The expression of PTEN, respectively is: A(6.44 + 6.44) %,(16.06 + 4.26) %B, C(29.82 + 2.22) %, D(62.46 + 4.36) %, increasing expression and was statistically significant; The expression of CD44V6 is respectively: A(80.48 + 80.48) %,(65.74 + 6.34) % B, C(48.88 + 9.28) %, D(28.70 +8.10) %, expression of diminishing and was statistically significant;Groups the expression of survivin, respectively is: A(84.64 + 84.64) %,(69.66 + 9.46) % B, C(31.3 + 9.50) %, D(18.42 + 4.62) %, expression of diminishing and was statistically significant; Groups of caspase 3expression is as follows: A(10.82 + 10.82) %,(15.24 + 4.64) % B, C(31.26 + 4.76) %, D(77.58 + 6.28) %, increasing expression and have statistical significance. SYK/HER2, PTEN/CD44V6, Survivin/caspase 3correlation analysis: as HER2, CD44V6, Survivin expression results,gradually decline, while the SYK, PTEN, caspase 3 gradually rise.Conclusion: cisplatin implants implanted on gastric cancer nude mouse transplantation tumor growth had a significant inhibitory effect, itspossible mechanism in inhibiting tumor growth is by raising SYK cut HER2 and to achieve a goal; Second, after raising PTEN expression and cut CD44V6 expression to achieve the purpose of inhibiting tumor growth, by cutting CD44V6 expression to reduce force of tumor and tumor of the process of the three obstacles to the late transfer process to achieve the effect of block tumor cell metastasis; 3 it is through the caspase 3 expression, the expression of Survivin cut to achieve the purpose of inhibiting tumor growth, make the expression of Survivin cut can weaken the inhibitory effect of caspase 3 expression, made in the process of cell apoptosis plays an important role in the final effect of protease caspase 3 can give full play to its role and achieve the effect of accelerating the apoptosis of tumor cells. Intervention through the above three kinds of mechanisms of gastric cancer cell growth, apoptosis,invasion and metastasis and prognosis, thus inhibiting tumor growth, due to tumor cells has a great power, and can carry on the theoretical guidance to the clinical practice, and it can significantly inhibit the growth of tumor, this advantage to the clinical treatment of gastric cancer research put forward some new targets and new ideas.