The Study On Different Methods Of Regulating The Stress Response To Acute Traumatic Effect

Objective: Observe and compare the inhalation anesthesia, spinal anesthesia, inhalation anesthesia combined with body acupuncture, auricular composite inhalation anesthesia, spinal anesthesia combined with body acupuncture, spinal anesthesia combined stress in rats with acute ear for stress hormone levels and hypothalamic / Impact spinal c-fos protein secretion, and to explore the possible mechanisms and regulation of clinical acute stress finding more effective treatments to provide a theoretical basis. Methods: Experiment1 Fifty male SD rats weighing 280~320g were used in this study, were randomly divided into 5 groups(n=10 each):Normal group(Ⅰ group), model group( Ⅱ group), sevoflurane group( Ⅲ group), sevoflurane combined with body acupuncture group( Ⅳ group) and sevoflurane combined with auricular acupuncture group(Ⅴ group). In addition to groupⅠ, other groups prepared as docked tails model, group Ⅰ placed in a glass case containing mixture gas within 30% O2 and 70% N2 in(flow 2L/min), group Ⅲ, Ⅳ and Ⅴ were placed in the 2% sevoflurane anesthesia box(flow 2L/min), Ⅳ group continued to stimulate "Zusanli" for 30 min once,4 times at intervals of 30 min,(stimulation parameters 3V, 3 Hz, 2 ms), Ⅴ group to stimulate "Apex", the same stimulation parameters as Ⅳ group. To measure serum corticosterone(CORT) and adrenocorticotropic hormone(ACTH) levels by ELISA with peripheral serum. Determination of c-fos protein expression levels withhypothalamic tissue by western bloting. Experiment 2 Fifty adultmale Sprague-Dawley rats weighing were randomly divided into 5 groups(n=10each) : group Ⅰ normal animal received no treatment only received IT normal saline 50μl; group Ⅱ docked animal received intrathecal(IT) normal saline50μl;group Ⅲ docked animal received IT fentanyl 50μl,2.5μg;group Ⅳdocked animal received IT fentanyl 50μl and EA at ‘zu-san-li’for 30 min(intensity 2m A,frequency 2/100 Hz);group Ⅴdocked animal received EA at‘er xue’ and IT fentanyl as in group Ⅲ and Ⅳ.To measure serum corticosterone(CORT) and adrenocorticotropic hormone(ACTH) levels by ELISA with peripheral serum. Take L4-6 spinal cord, determination of c-fos protein expression levels with the organization of spinal by western bloting,analyzed by IPP6.0 imaging system.Results :① Acute stress after docked before SD rat peripheral blood serum ACTH and CORT was increased compared with not docked ② Electrical stimulation "Zusanli" serum rat tails ACTH and CORT levels of value in varying degrees of reduction, the difference was statistically significant(P <0.05). ③EA complex after sevoflurane anesthesia treatment tails stress and pain after only using sevoflurane anesthesia compared to EA composite sevoflurane anesthesia downward effect on peripheral blood serum levels of ACTH and CORT more obvious(P <0.05), the effect is to alleviate the stress more significant. ④Within EA complex spinal anesthesia fentanyl pain after treatment tails stress than simply using intrathecal fentanyl stronger role,Conclusion:①Body acupuncture and auricular composite electrical stimulation were intrathecal anesthesia to reduce pain in rats with acute stress reaction force;under intrathecal anesthesia, regional nerve block Ear role of non-obvious,which may be related neurotransmitter release evoked spinal cord c-fos proteins.② EA "Zusanli", " Auricular" complex sevoflurane anesthesia can reduce the stress response in rats with acute trauma, and simple regulation of sevoflurane anesthesia enhanced role; sevoflurane complex stimulus " Auricular" when a strong regulatory role of stress in the composite stimulus " Zusanli." Acupuncture composite adjustment to stress by regulating the hypothalamic paraventricular nucleus c-fos protein secretion achieve.