| Objective: To analyze the role and correlation of Axl and Gas6 during the development of papillary thyroid carcinoma (PTC) by detecting the expressions of Axl and Gas6 in the PTC tissues, nodular goiter (NG) tissues and normal tissues adjacent to the tumors as well as their serum concentration in the PTC patients, NG patients and normal adults.The study also aims to explore the practicability of Axl and Gas6 as molecular markers of the thyroid carcinoma, and provide the new idea to the early diagnosis and prognosis of thyroid carcinoma.Methods:1.Cases collection and grouping:54 cases of PTC specimens and blood (PTC group),16 cases of nodular goiter specimens and blood(NG group),70 cases of normal tissues adjacent to the tumors and 10 cases of healthy persons (N group) with integrity clinical pathological datas were collected from January 1,2013 to January 1,2014 year.Those specimens were resected by First Affiliated Hospital of Hebei North University operation and tested by pathology diagnose.2 The expressions of Axl and Gas6 proteins in PTC, NG and normal tissues adjacent to the tumors were detected by Western blot and immunohistochemical SP method, and the relationships with the clinical pathology characteristics were analyzed combined with the integrity clinical pathological datas. The transcriptional level changes of Axl mRNA and Gas6 mRNA were tested by real-time reverse transcription polymerase chain reaction.3 The concentrations of Axl and Gas6 in the serum of PTC patients, NG patients and normal adults were detected by enzyme linked immunosorbent assay(ELISA) method, and the relationships between the serum level and clinical pathology characteristics of thyroid papillary carcinoma were explored.4 All the statistical analyses were carried out using SPSS 17.0. The datum were expressed as x±s and analyzed by ANOVA analysis and Q test.Correlation analysis between Axl and Gas6 applied Spearman non parametric correlation analysis. The positive rates of immunohistochemistry were compared by chi square test. Bilateral p<0.05 was considered statistically significance.Results:1 The results of immunohistochemistry staining showed Axl proteins were expressed in different strength in all PTC tissues. Axl positive proteins were mainly located in the cancer cell membrane, A few were found in the cytoplasm. In NG and normal tissues adjacent to the tumors Axl expression was weakly positive or negative.Gas6 was mainly expressed in PTC cells cytoplasm, and negative expression in NG and normal tissues adjacent to the tumors. Axl and Gas6 positive expression rate in PTC was significantly higher than in NG and normal tissues adjacent to the tumors (p <0.01) and whose expressions were no significant difference between in NG and normal tissues adjacent to the tumors (p> 0.05). The abnormal expressions of Axl and Gas6 were unrelated to the age, sex and size of the tumor (p> 0.05) of PTC patients, but associated with the lymph node metastasis and pathological stage (p< 0.05).2 The results of Western Blot showed the Axl and Gas6 proteins in PTC tissues were significantly more than in NG and normal tissues adjacent to the tumors with statistical significance. (p<0.05), but no statistical difference between in NG and normal tissues adjacent to the tumors (p> 0.05).3 The results of Real-time PCR showed the transcriptional expression levels of the Axl mRNA and Gas6 mRNA in PTC tissues were significantly higher than in NG and normal tissues adjacent to the tumors with statistical significance(p<0.05), but no statistical difference between in NG and normal tissues adjacent to the tumors (p>0.05).4 The results of ELISA showed the concentrations of Axl and Gas6 in the serum of PTC patients were significantly higher than of NG patients and normal adults with statistical significance.(p<0.05), but no statistical difference between in the serum of NG patients and normal adults (p>0.05). The concentrations of Axl and Gas6 in the serum of PTC patients had no relation to the age, sex and the size of tumor(p>0.05),but to the lymph node metastasis (p< 0.05).5 There was positive correlation between expression of Axl and Gas6 protein in PTC(r=0.358, p=0.015).Conclusion:1 Axl and Gas6 are all detected to exist in PTC and NG tissues,whose expressons are stronger in PTC tissues than in NG.The higher expressions of Axl and Gas6 have the positive correlation with the lymph node metastasis and clinicopathologic stage and the positive correlation are also existed between the expression of Axl and Gas6,which play the important role in the process of PTC, include the occurrence, development,invision and metastasis.2 The serum concentrations of Axl and Gas6 in PTC patients are higher than in NG patients and normal adults,and have the positive correlation with the lymph node metastasis, serologic examination of both are regarded as independent detection indicators. |
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