The Infulence Of Polyethyleneimine On The Conformation Of Bovine Serum Albumin And Lysozyme

Nowadays, Gene carrier material is one of the most popular research areas, the development of gene therapy technology plays an inestimable role toward it. Gene carrier material, however, the study of biological safety is relatively lag, theory is not yet mature. This article chooses the current relatively common genetic carrier materials of polyethyleneimine, Once upon a time, few people pay attention to the molecular level study of the biological safety, the influence of polyethyleneimine towards the conformation of lysozyme or bovine seru M albμMin can help the study of the mechanism of biological safety.the main results were as follows:PEI combines with Lys and BSA can occur indeed through direct interaction, and form complexes.Alpha helix and beta folded of BSA and Lys have little influence by PEI upon secondary structure, and PEI mainly influence tertiary structure of Lys and BSA.The obvious difference of molecular weight and concentration of PEI has significant influence on the conformation of BSA and Lys. The greater concentration, the higher molecular weight of PEI have greater influence on the two kinds of protein tertiary structure, forming larger complex particle size and higher zeta potential.It is easier to make blue shift phenomenon appears, and it has a relatively obvious compression effect towards BSA when PEI have larger molecular weight. Smaller molecular weight and concentration of PEI has small influence on protein tertiary structure.Quercetin and BSA quenching of the experimental results show that PEI’s influence on the function of BSA carrier is obvious, and macromolecular PEI has more obvious influence. PEI affect the delivery function of BSA by affecting the structure of BSA.