| Objective:The purpose of this study was to investigate the relevance between the expression and clinicopathological parameters of mi R-200 c and E-cadherin in gastric cancer, corresponding adjacent gastric tissue and normal gastric mucosa tissues, so as to better understanding the role of mi R-200 c and E-cadherin in development and progression of gastric cancer, hence to offer theoretical grounds for developing new methods and new drugs for the treatment of gastric cancer.Methods:(1) Tissue samples were obtained from 30 cases of surgical gastric cancer organizations and their corresponding adjacent gastric tissue, and another 30 cases of normal gastric mucosa tissues of healthy volunteers by gastroscope.(2) The RT-q PCR and immunohistochemical were used to assess the relative expression of mi R-200 c and E-cadherin in gastric cancer tissue, corresponding adjacent gastric tissue and normal gastric tissues.(3)Spss18.0 statistical software was applied to test data, inspection level of a=0.05 and P<0.05 were considered to be significant. The nonparametric analysis and the method of Spearman were respectively employed to analyze the relative quantitative and relevance of mi R-200 c and E-cadherin.Results:(1) The expression of mi R-200 c were 0.57±0.96,1.23±0.99 and 1.32±1.02 in gastric cancer tissue, corresponding adjacent gastric tissue and normal gastric tissues on average. There was a statistical difference(P<0.05) between gastric cancer tissue and corresponding adjacent gastric tissue,and also a statistical difference(P<0.05) between the gastric cancer tissue and normal gastric tissues, while no statistical difference(P>0.05) between corresponding adjacent gastric tissue and normal gastric tissues.(2)The expressions of mi R-200 c in low(13/30) and high(17/30) histologic patients were 0.19±0.28 and 0.87±1.18 on average, which means that the expression of mi R-200 c on average was closely related(P<0.05)with the level of differentiation, but was irrelated with age, size of tumor, histologic, TNM stage and lymph node metastasis(P>0.05)(3)The positive expressions of E-cadherin each of gastric cancer tissue, corresponding adjacent gastric tissue and normal gastric tissues were 33.33%(10/30), 90%(27/30)and 100%(30/30)by immunohistochemical test. There was a statistical difference(P<0.05) between gastric cancer tissue and corresponding adjacent gastric tissue and also a statistical difference(P<0.05) between gastric cancer tissue and normal gastric tissues, but no statistical difference(P>0.05) between corresponding adjacent gastric tissue and normal gastric tissues.(4)The positive expressions of E-cadherin each were 52.94%(9/17)and 7.69%(1/13)in high and low histologic, 57.14%(8/14) and 12.50%(2/16)in stage of I~II and III~IV, 14.29%(3/21)with lymph node metastasis and 77.78%(7/9)with no lymph node metastasis, and the differences were all significantly related(P<0.05),and were irrelated with age and size of tumour(P>0.05).(5)The expressions of mi R-200c and E-cadherin were positively correlated in gastric cancer tissues(r=0.416, P<0.05).Conclusions:(1) The expressions of mi R-200 c and E-cadherin in gastric cancer tissue is low, which hints that mi R-200 c and E-cadherin may negatively correlated with the development and progression of gastric cancer.(2) The expressions of mi R-200 c and E-cadherin in different differentiation degree of gastric cancer were variant, which prompts that both of them are associated with the differentiation degree of gastric cancer;The expressions of E-cadherin are different in each TNM stage, and also different in lymph node metastasis and no lymph node metastasis of gastric cancer tissue, which prompts that E-cadherin is associated with TNM stage, and lymph node metastasis of gastric cancer.(3)The positive correlation between the expression of mi R-200 c and E-cadherin, which hints that the mi R-200 c may control the expression of E-cadherin through an specific signaling pathway, so as to realize the regulation of biological characteristics of gastric cancer. |
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