| Objective:To investigate the regulatory effects of probiotics on Toll-like receptors (TLRs) expression in an ulcerative colitis (UC) rat model, and to discuss the role and possible mechanisms of probiotics in the development of UC.Methods:Rats were assigned into the healthy control, model, Golden bifid treatment and TLR4mAb intervention groups. UC rat model was established using 2,4,6-trinitro-benzene sulfonic acid (TNBS). Rats’general status and histological changes were scored using the disease activity index (DAI) and the histopathological scoring (HPS) method, and their expression of TLR4/TLR2 were measured using RT-PCRUsing ELISA to detect the concentration of TNF-a/IL-1 p in serum.Result:The expression of TLR4/TLR2 in colon tissue and concentration of TNF-a/IL-1β in serum of model group was significantly higher than the healthy control, but rats received Golden bifid treatment or TLR4mAb intervention exhibited significantly decreased (P< 0.05) level of TLR4/TLR2 mRNA comparing to the model rats.Conclusion:The development of UC is associated with abnormal intestinal immune response. Probiotics alleviated inflammatory reactions in rats with UC, and the mechanisms might be associated with the expression of TLRs as well as the inflammatory cytokines. |
PDF Full Text Request