| Objective: To explore the influence of pretreatment with huperzine-A to the learning and memory of neonatal rats anesthetized by sevoflurane. Methods:Eighty male and female 7-day-old SD rats that weighed 15-20g, were randomly divided into five groups (n=16):control group (group C), group anesthetized by 3% sevoflurane for 2h (group S1), group pretreated by huperzine-A and anesthetized by 3% sevoflurane for 2h (group AS1), group anesthetized by 3% sevoflurane for 6h (group S2), and group pretreated by huperzine-A and anesthetized by 3% sevoflurane for 6h (group AS2). For the groups with pretreatment, huperzine-A was intraperitoneally injected by the dosage of 0.2mg/kg 30min before anesthetization. After 24h of the end of anesthetization, eight rats were randomly sampled from each group and decapitated for blood; ELISA method was used to detect the concentrations of serum S100β protein and neuron-specific enolase (NSE). The rest rats in the five groups underwent the Morris water maze when they were 14 days old to determine their learning and memory. Results:Compared with group C,1st DAY-6th DAY escape latency, platform quadrant retention time and times of crossing the platform of group AS1 showed no significant change; the escape latency of group AS2 was prolonged (P<0.05), while its platform quadrant retention time and times of crossing the platform showed no significant change; the serum S100β and NSE concentrations of all rest groups of rats were increased (P<0.05). Compared with group S1,1st DAY-6th DAY escape latency of group AS1 showed no significant change, while its platform quadrant retention time and times of crossing the platform were increased (P<0.05), and the serum S100β (73.9±26.9)pg/ml and NSE (6.0±2.0)ng/ml concentrations were reduced (P<0.05). Compared with group S2,1st DAY-6th DAY escape latency, platform quadrant retention time and times of crossing the platform of group AS2 were improved (P<0.05), and the serum S100 β(76.1 ± 18.3)pg/ml and NSE (7.2 ± 3.6)ng/ml concentrations were reduced (P<0.05). Conclusion: The pretreatment with huperzine A can effectively improve the learning and memory of neonatal rats anesthetized by sevoflurane and plays certain protective role for nerve cells. |
