Adjuvant Activity Of Ginsenosides Rb1, Rb2 And Rg3 In DNA Vaccines

Araliaceae Ginseng is a perennial herb, the main active ingredient of ginseng saponins and ginseng polysaccharides. Since ancient times, Chinese medicine ginseng is blindly magic, with a sedative, adjust blood pressure, improve the body immunity. In recent years, many studies have found that ginsenosides can regulate the immune response, and immunogenicity of DNA vaccine is relatively insufficient, therefore, this paper studies the ginsenosides Rb1, Rb2 and Rg3 as a DNA vaccine adjuvant activity.In this experiment, respectively ginsenoside Rb1, Rb2 and Rg3 as an immune adjuvant and DNA vaccine co-immunized C57 BL / 6J mice, while mice immunized with pc DNA3.1 and PBS as the control group. After inoculation intramuscular electroporation, two immunizations two weeks apart, two weeks after the second immunization, mice were eyeball collection of serum to detect qualitatively Western Blot PBS, pc DNA3.1, p D-HSP90 C, p D-HSP90 C / Rb1, p D-HSP90 C / Rb2, p D-HSP90 C / Rg3 in the serum antibody specificity; and by ELISA and ELISA kit quantitatively detect levels of serum antibody titers and cytokine concentration.The results showed that: compared with the p D-HSP90 C group, p D-HSP90 C / Rb1 and p D-HSP90 C / Rg3 groups were produced higher Ig G, Ig G1, Ig G2 b antibody titers and cytokine IL-2,4, and Rb2 no significant DNA vaccine adjuvant effect in p D-HSP90 C.In this paper, experimental results show that ginsenoside Rb1, Rg3 effective in promoting Th1 and Th2 type immune response, and Rb2 no significant effect, laid the foundation for the development of ginseng saponin adjuvant in the vaccine field.