Expression Of Heparanase MRNA And Its Relation With The Pathological Features In Breast Carcinoma

Background Heparanase is an endo-β-glucuronidase, which is capable of cleaving heparan sulfate proteoglycan in a particular part, led to the extracellular matrix and basement membrane degradation, promote tumor invasion and metastasis and angiogenesis. Breast carcinoma is one of multiple malignant tumors in our country. To our knowledge, HPA mRNA expression in breast carcinoma and its relationship with the invasive growth, lymph node metastasis and angiogenesis in breast carcinoma have not been evaluated so far. Expression of HPA mRNA in 50 breast carcinoma tissues and adjacent normal breast tissues were detected by RT-PCR assay, HPA mRNA expression were analysed with their relation to invasion depth, metastasis and microvessel density(MVD), Now report as follows.Objective Expression of heparanase mRNA and its relation with the pathological features and MVD in breast carcinoma.Methods Collect 50 cases of breast carcinoma tissues samples and 50 adjacent normal breast tissue samples(5cm from the edge of the tumor) after the surgical treatment in First Affiliated Hospital of Xinxiang Medical College from February 2013 to September 2013. Their are all female patients in this 50 tissues, the age is between 23 to 76 years, and the average age is 56 years old.50 patients were not receiving preoperative radiotherapy, chemotherapy and endocrine therapy before operation. Postoperative routine pathological section all confirmed as breast carcinoma. According to the Union for International Cancer Control(UICC) version 7"The Breast TNM Staging Standard", there are 14 well-differentiated cancers cases and 26 middle differentiated cancers cases and 10 poor differentiated cancers cases; 10 cases of Ⅰ group and 24 cases of Ⅱ group and 16 cases of Ⅲ group; 18 cases of NO,20 cases of N1 and 9 cases of N2 and 3 cases of N3. Each sample was divided into 2 parts, one part of specimens are put into liquid nitrogen to preserve for the extraction of RNA; the others are fixed by 40 g-L-1 paraformaldehyde and then paraffined after biological automatic dehydration machine processing for immunohistochemistry. Expression of heparanase mRNA in 50 breast carcinoma tissues and adjacent normal breast tissues were detected by RT-PCR assay, inaddition, CD34 was detected by immunohistochemistry. SPSS 19.0 statistical software was used to statistical analysis, measurement data was represented by mean±tandard deviation(x±s), Pairwise comparison was performed with Student t test. Count data comparison was performed with Chi square test. Statistical analysis examination standard a=0.05.Results Comparative analysis through the statistical, positive rate of heparanase mRNA in breast carcinoma tissues 70.0%(35/50), significantly higher than that in the adjacent tissues 24.0%(12/50)(P<0.01); expression of HPA mRNA was positively correlated with histological grading, lymph node metastasis, TNM staging and serosal invasion in breast carcinoma. (P<0.05,P<0.01), but irrelevant with the patient’s age(P>0.05); CD34 mainly expressed in membrane and cytoplasm of artery endothelial cell, presented as brownish yellow, MVD in heparanse mRNA positive group (58.20±18.56) was significantly higher than that in heparanase mRNA negative group (41.30±9.35,P<0.01).Conclusion HPA mRNA expression was significantly higher in breast carcinoma, has statistical significance compared with adjacent tissues. MVD in heparanse mRNA positive group was significantly higher than that in heparanase mRNA negative group. The high expression of heparanase can promote the invasion, metastasis and angiogenesis of breast carcinoma, clearly indicating that heparanase can be an objective indicator of the biological behavior of breast carcinoma and as a basis for clinical tumor selection or to forecast the patient’s prognosis.