Effects Of The Peach Blossom Polyphenols On Related Proteins And Memory Ability In AD Rat

Background Alzheimer’s disease(AD) is a kind of irreversible brain function declining disease, manifesting as mainly progressive cognitive impairment and memory damage central nervous system degenerative disease. AD is a common mood disorder. The physiopathologic mechanism is complicated, and the pathogenesis is not clear so far. AD is seriously affecting human health and living quality. AD disease, also called Alzheimer’s disease, that is what we usually called senile dementia or presenile dementia. So far, there is no effective way to cure and interdict the disease. Therefore, once suffered from AD, there is no doubt that one can only wait for brain function declining until death.Objective Discuss the impact of peach blossom Polyphenols on the Behavioral Memory Ability and Interacting Protein on the Model Rats with Alzheimer’s Disease.Methods Select 50 health adult rats. The rats take food freely, drink 1% water with sucrose 3d, the original drinking habit of the rats was changed. The rats were intraperitoneal anesthetized by 10% chloral hydrate. The injection point was determined 3.5mm behind the bregma,2mm to the left of midline.40 rats are used for molding. 2μL(10μg) Aβ1-40 was slowly injected into the brain tissue using microsyringe.10 rats are used for contrast. The same volume of saline was administrated into the same point. Intramuscular injection of 400 thousand u/d penicillin for anti-injection for 5 days in a row. The rats take food and drink water freely. Observe for two days. Then the MORRIS water maze test was carried out. Calculate the mean escape latency value of the Model animals. If the difference value between the escape latency value of each rat and the mean escape latency value to the mean escape latency value ratio >20%, shows positive molding. Randomly select 30 rats from the positive molding animals. These 30 rats were randomly divided into Model group(MG), PBP-L dose group(20mg/kg), PBP-H dose group(80mg/kg). These rats were intragastric administrated 1 time everyday for 28 days in a row. The rats in Shams group(SG) and MG rats were administrated equivalent distilled water. The behavioral change of rats was detected by water maze test. The expression levels of PSD-95 protein and S100β protein molecule immunohistochemistry and immunofluorescence antibody were detected by water maze test.Results Water maze test showed, the seek time in the target quadrant of Model group, that is the escape latency, was significantly prolonged compared with the Sham group. The escape latency of PBP-L and PBP-H intervention group was significantly reduced compared with Model group(P<0.05). Compared with animals in Sham group, space exploration competence in the target quadrant of animals in Model group was significantly declined, they swam less times in platform quadrant crossing in unit time, and reduction of the time staying in target quadrant was statistically significant(P<0.01); the duration of stay in platform quadrant of intervention groups PBP-L and PBP-H were longer, and the rats swam more times in platform quadrant crossing than in Model group. The times that the rats swam crossing platform quadrant in unit time was significantly increased(P<0.01). Compared with animals in normal contrast group, total antioxidant capacity and SOD activity of brain tissue of animals in Model group declined, MDA content increased(P<0.01); while compared with Model group, total antioxidant capacity and SOD activity of that of PBP-L and PBP-H intervention groups were significantly increased, MDA content was significantly declined(P<0.01). Immunohistochemistry results showed, a few PSD-95、S100-β positive immunohistochemistry cells were visible, the cells were stained lighter; in Model group, the protein expression of PSD-95、S100-β were detected at high levels, the cells were stained darker, particles were enlarged, the OD value was significantly different compared with that of Sham group(P<0.01); PSD-95、S100-β positive cells expression of PBP-L and PBP-H groups declined compared with Model group, meanwhile cell staining OD value declined compared with Model group(P<0.01); we have observed by fluorescence microscope that PSD-95 protein and S100-β protein expression positive cells presented gathered or sparsely localized in the hippocampal dentate gyrus, while in Sham group the green fluorescence cells were significantly fewer than that of Model group(P<0.01). Positive cell expression of PBP-L and PBP-H intervention group declined compared with that of Model group, the difference was statistically significant(P<0.01). Western blot test found that PSD-95、S100-β protein expression levels of Model group were significantly increased compared with that of Sham group(P<0.05); PSD-95 、S100-β protein expression of PBP-L and PBP-H groups were significantly decreased than that of Model group(P<0.05). Hippocampus in Model group was observed condensing around the nucleus by transmission electron microscope, presenting crescent cells. Karyotheca was damaged. There was a mass of lipofuscin cell membrane structure in the cells. Electron density of chromatin enhanced. Cytoplasmic hypervacuolization. Cell membrane ruptured. Disintegration was observed, forming typical character of apoptosis. Cytopathology change of PBP-L and PBP-H groups were significantly lightened compared with Model group. Hippocampus CA1 dysplastic neurons were significantly decreased compared with Model group.Conclusion(1) Peach blossom polyphenols have a good antioxidant ability and can improve the learning and cognitive defect of AD rats;(2) Peach blossom polyphenols can reduce the expression of PSD-95、S100-β in hippocampus. This may be one of the neuroprotection and anti-AD mechanisms.