The Study Of The BMP-6 On The Process Of Rat Bone Marrow Mesenchymal Stem Cells Osteogenesis Under Stress Tensile

Objective:To evaluate the impact of bone morphogenetic protein(BMP)-6 on the process of rat bone marrow-derived mesenchymal stem cells(BMMSCs) osteogenesis under tensile stress.Methods:Different tensile stress was applied on rat BMMSCs by four-point bending apparatus for 60 minutes. Two hours later, the change of the BMP-6 m RNA level and alkaline phosphate, osteocalcin and runt-related transcription factor 2 m RNA level were also examined. Then under the poor osteogenesis condition(4000μstrain), the BMP-6 adenovirus transfected BMMSCs, blank adenovirus transfected BMMSCs and control BMMSCs were also under the same testing to evaluate the osteogenic ability.Results: With the increase of tensile stress, the expression of BMP-6 was gradually increased, so was the osteogenic capacity. However, both of the BMP-6 expression and osteogenic capacity decreased obviously after the tensile stress exceeded 2000μstrain. Under the 4000μstrain stress tensile, the transfected cells presented better osteogenic ability than control cells.Conclusion: 1.The proper tensile stress would promote BMP-6 expression and BMMSCs osteogensis. 2.The BMP-6 would recover the compromised osteogenic ability by major stress tensile.