The Influence Of Phenotypiccontractile Proteins In Human Bladder Smooth Muscle Cells During Short Duration Of Continuous Extension And The Relationship With Erk/MAPK Signal Pathways

Objective:To investigate the changes of contractive phenotypic proteins in human bladder smooth muscle cells during short duration of continuous extension and the relationship with Erk/MAPK signal pathways.Methods:In our experiment, Real-time PCR and Western blot were used to measure the changes of contractive phenotypic proteins at the level of mRNA and protein. Immunology and Immunofluorescence were used to observe the changes of a-SMA when influenced by MAPK inhibitions. Analysis the changes of contractive phenotypic proteins in human bladder smooth muscle cells during short duration of continuous extension and the relationship with MAPK signal pathways.Results:After continuous stretch of human bladder smooth muscle cells, a-SMA first decreased and then increased unsuspectedly., which is closely related to the cell morphology.After reliving extension, α-SMA could still increase further. During the pulling process, Erks appeared to change significantly, while P38 had no obvious change. When the Erk pathway or P38 pathway was blocked by inhibitor, the former showed significant changes in contractive phenotypic proteins in human bladder smooth muscle cells.Conclusions:α-SMA and cell morphology can reflect the ability of human bladder smooth muscle cell’s contraction. The contractility was first shown as decrease then increase. After relieving of extension, its contractility still increases, which is related to the Erk pathway and has nothing to do with the P38 pathway...