Establish A Live Metastasis Model With High Transfer And Invasive From Colon Cancer

Objective:This article first establish the optimum condition for a colon cancer xenograft model through the packet filtering optimumconditions, and then changing the traditional method by uses xenograft tumor cells with splenectomy to establish animal model of colon cancer liver metastasis. Methods:According to different cell media and the injection site, the HT29 human colon cancer cell lines were washed with PBS and resuspended in serum-free medium after injection in BALB / C mice armpit and neck skin. Build first-generation xenograft model, as a xenograft source, using interpolation block method, and homogenized enzymatic method. A second-generation xenograft model, choose the most preferred method of screening after the establishment of the third generation of subcutaneous xenograft model, and establish colon cancer liver metastases model with spleen injection and excisioned method, observate the tumor growth and metastasis, and histopathology, flow cytometry technique to test the transplanted tumor. Result: The success of established operability, high repeatability colon cancer xenograft model observed tumor biopsy showed that the tumor was adenocarcinoma Ⅰ～Ⅱ grade. Immunohistochemistry showed positive expression of CK19 and P53 flow cytometry analysis of DNA ploidy check aneuploid. Conclusion:The study successfully established an appropriate colon cancer screening and xenograft model using human colon cancer cell line HT29 with BALB/C mice.In addition, it provided an excellent technical platform for the next step of colon cancer metastasis model of biological research.