| Iron is an important element of body, the transfer of oxygen, participating in the process of DNA synthesis, the redox reaction of cellular respiratory chain and so on. However, the abnormity of iron homeostasis could lead to the disfunction of the body. Ferritin increases as estrogen decreases in the postmenopausal women. Recently many studies have shown that: estrogen level of female declines with skin aging, and iron accumulation may be another risk factor in postmenopausal women with intrinsic aging. So not only the decrease of estrogen but also the iron accumulation lead to skin aging and lowering iron accumulation was suggested as a strategy for aging.Therefore, it is essential to create the animal model which imitating the estrogen decrease and iron accumulation. We use the ovarietected rats to imitate postmenopausal intrinsic skin aging, and the iron accumulation conditions were established by the intraperitoneal injection of ferric ammonium citrate(FAC) as iron donor, and we use iron chelators to be another intervention group, then observe the changes of serum estrogen, ferritin, the iron content of skin, thickness of dermis, and thickness of collagen. The purpose is to further explore the effect of iron overload related mechanism of intrinsic skin aging, so as to solve the problem of aging skin fundamentally and to provide new ideas for the treatment of related diseases.Objective: Use the ovarietected rats to imitate postmenopausal intrinsic skin aging, and the iron accumulation conditions were builded by the intraperitoneal injection with ferric ammonium citrate(FAC) as iron donor, then use deferoxamine(DFO)to intervene the station of iron accumulation, observe the changes of serum estrogen, ferritin, the iron content of skin, thickness of dermis and collagen. Then explore the effect of iron accumulationon in intrinsic skin aging under postmenopausal conditions.Methods: 32 three-month-old female SD rats were randomly divided into 4 groups(n= 8 each): SHAM group,OVX group and iron accumulation group(FAC group), ferric ammonium citrate and deferoxamine intervention group(FAC+DFO group). In FAC intervention group, FAC group were injected by the FAC with 180mg/Kg twice a week for 12 weeks after one week of the operation; FAC+DFO group were injected by FAC 180 mg/Kg and DFO 30mg/Kg with the same dose. The other groups were injected by the same dose of 0.9% normal saline for 12 weeks.12 weeks later,serum estrogen,serum ferritin were measured by peripheral blood. The iron content of the left back skin was measured by ICP tester. The thickness of dermis and collagen of the right back skin were measured by HE and MASSON.Results:1. Serum estrogen: serum estrogen level of OVX group 16.46±0.71 ng/L, FAC group 17.06±1.06 ng/L, FAC+DFO group 16.66±0.80 ng/L was significantly lower than that of SHAM group 32.82±1.37 ng/L(P<0.05).2. Serum ferritin: the content of serum ferritin in OVX group was 1105.82 + 76.19 ug/d L and the SHAM group was 1161.06 + 91.35 ug/d L, no significant difference(P>0.05), FAC group 3318.27 + 109.69 ug/d L, FAC+DFO group1437.61 + 144.80 ug/d L were higher than that of OVX group, the differences were statistically significant(P<0.05); FAC+DFO group of serum ferritin levels were lower than the FAC group(P<0.05).3. Iron content of the back skin :The iron content of back skin in OVX group 63.77±3.21 ug/g and the SHAM group 62.91±1.86 ug/g no significant difference(P>0.05), FAC group 85.34±10.26 ug/g, FAC+DFO group 70.00±4.39 ug/g were significantly higher than OVX group 62.91±1.86 ug/g(P<0.05). The iron content of back skin in FAC group were higher than FAC+DFO group(P<0.05).4. Thickness of dermis in back skin: The thickness of dermis in back skin in OVX group 0.615±0.004 mm,in FAC group 0.456±0.006 mm,in FAC+DFO group 0.532±0.054 mm were significantly thinner than SHAM group 0.733±0.078mm(P<0.05).Compared with OVX group, the thickness of dermis in back skin in FAC group and FAC+DFO group were thinner(P<0.05) and FAC+DFO group was thicker than FAC group(P<0.05).5. Thickness of collagen in back skin : The thickness of collagen in back skin in OVX group 0.533±0.272 mm,in FAC group 0.443±0.210 mm,in FAC+DFO group 0.605±0.0190 mm were significantly thinner than SHAM group 0.605±0.019mm(P<0.05).Compared with OVX group, the thickness of collagen in back skin in FAC group and FAC+DFO group were thinner(P<0.05) and FAC+DFO group was thicker than FAC group(P<0.05).Conclusion: Iron accumulation could lead to the decreasing of thickness of dermis and content of collagen in OVX rats,a certain dose of DFO may reduce the content of serum ferritin, iron content of the back skin,which were lower than the normal control group. Suggesting that intrinsic skin aging after menopause occured with iron accumulation, DFO can reduce status of the iron accumulation, at the same time, improve the skin aging. |
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