Discussion And Analysis Of Results Of Second Trimester Amniocentesis For Prenatal Diagnosis Indications

Objective: To explore the relationships of the safety amniocentesis and the detection rate of abnormal karyotype with different indications of prenatal diagnosis of 2911 women in six years.Methords: From July 2008 to December 2014 in Yancheng MCH prenatal diagnosis center, 2911 cases pregnant women with indications of prenatal diagnosis, performed amniocentesis positioned by the B ultrasound, culture and cytogenetic analysis extracted 20 m L amniotic fluid. Analysis different abnormality detection rate and distribution of indications of prenatal diagnosis of chromosomal, security of amniotic fluid puncture, to provide an objective basis for experimental prenatal genetic counseling.Results: 1. 2911 cases pregnant women performed amniocentesis in line with indications of prenatal diagnosis. 2906 cases punctured successfully, puncture success rate was 99.82%; 2902 cases cultured chromosomes successfully, the culture success rate of 99.86%; 3 cases aborted by followed up after puncture, abortion rate was 0.10%.2. 91 cases were found abnormal karyotypes, the detection rate was 3.14%. 35 cases were found 21 trisomy, accounting for 38.46%; 5 cases were found 18 trisomy, accounting for 5.49%; 8 cases were found sex chromosome abnormality, accounting for 8.79%; other abnormal number of chromosomes 5 cases, accounting for 5.49%;38 cases were found chromosome structural abnormality(including inversion and translocation etc.), accounting for 41.76%.3. Abnormal rate of high-risk Down’s screening were 2.97%, abnormal rate of senile pregnant women were 2.44%, abnormal rate of adverse pregnancy history were 3.77%, abnormal rate of ultrasonic predicting abnormal were 30.0%, abnormal rate of pregnant women abnormal were 18.18%, abnormal rate of other abnormal indications were 6.25%.Conclusions:1. Abortion rate of amniocentesis is very low; culture successful rate is high, and it is relatively safe and feasible as a method of prenatal diagnosis. Prenatal diagnosis of complicated and arduous work, we should improve the technology to shorten the diagnostic cycle, reduce the failure rate of puncture and culture, and reduce the psychological burden of pregnant women and their families.2. They are necessary chromosome examination that high-risk Down’s screening, pre-production period over the 35 years old, adverse pregnancy history, abnormal ultrasonic prediction, chromosomal abnormalities of pregnant women and their husbands and other abnormalities such as viral infections in early pregnancy, maternal exposure to toxic and hazardous substances.3. At present, most of our pregnant women are still doing common Down’s screening, screening positive serum is the main reason for the amniocentesis. Thereby we need to improve the positive rate of Down’s screening is currently. We can take joint Down’s screening and standardized and accurate ultrasound screening.