The Effect Of Gastric Bypass On Expression Of Glycometabolism-related Factors In The Liver Of T2DM Rat

Objective We established type 2 diabetes-Gastric Bypass rat model, to explore the effect of GBP on expression of glycometabolism-related factors in the liver.Methods Twelve only six weeks old Rats were randomly divided into groups of normal chow diet(NCD), high-fat diet(HFD) and high-fat diet followed by GBP (HFD-GBP).4 weeks after high-fat diet, rats were injected with STZ intraperitoneally to induce type 2 diabetes. Then, we monitored body weight and fasting blood glucose concentration weekly, and examined peroperative glucose tolerance and insulin sensitivity in HFD rats.2 weeks later, we performed the GBP operation on HFD rats (HFD-GBP group). During the next 2 weeks, we examined and compared weight, fasting blood glucose, serum bile acid, total cholesterol the groups and triglyceride levels in the 3 groups. Total RNA and protein were extracted from the liver of three groups, and expression of FXR, PEPCK, G6Pase, GLUT-2 and FGF-21 was detected by Real-time-PCR and Western blotting.Results Compared with NCD and HFD group of rats, total serum bile acid level of the HFD-GBP rats significantly raised (p<0.05, p<0.01). Total serum cholesterol and triglyceride levels of HFD group were obviously higher than the NCD group (p<0.05; p<0.05) and compared with the HFD group, total serum cholesterol and triglyceride levels of HFD GBP group were significantly lower (p<0.05; p<0.05). Compared with the HFD group, weight of HFD-GBP group rats was decreased and blood glucose concentration was improved (p<0.05). In livers of HFD-GBP rats, FXR and GLUT2 mRNA expression significantly raised (p<0.05). Meanwhile, PEPCK, G6Pase and FGF-21 mRNA expression also increases. Compared with the NCD group, FXR and PEPCK protein expression significantly elevated (p<0.05) in HFD and HFD-GBP group rats.Conclusion GBP model can effectively improve glucose metabolism in obese T2DM rats. Total serum bile acid level of postoperative rats significantly raised.FXR, PEPCK, GLUT2 and FGF-21 may participate in blood glucose regulation.