The Expression Of Cancer Stem Cell Marker In Human Nasopharyngeal Carcinoma Cell Lines

Objective:Investigation of the expression of cancer stem cells(CSC) markers of ABCG2 、 CD44 、 CD34 in human nasopharyngeal carcinoma(NPC) CNE-2 、 5-8F 、 and 6-10 B cell line,and the Clone-forming capability of ABCG2+、CD44- and CD34+ cells of 5-8F. to choice a possible cancer stem cell marker for the research of NPC.Methods:Flow cytometry was used to investigate the expression of cancer stem cells of markers ABCG2、CD44、CD34 in CNE-2、5-8F、and 6-10 B. Clone-forming capability of ABCG2+、CD44- and CD34+ cells of 5-8F was determined by using softer agar clone formation assay.Results:ABCG2 positive cells accounted 0.1%、18.6%、19.9% in 5-8F 、6-10 B 、CNE-2 cell lines;CD44 positive cells accounted 99.5%、93.2%、99.1% in 5-8F 、 6-10 B 、CNE-2 cell lines;CD34 positive cells accounted 0.0 % 、 3.0 % 、 0.1 % in 5-8F 、 6-10 B 、 CNE-2 cell lines;Compared with ABCG2- cells, ABCG2+ cells show higher clone-forming capability. CD34+ cells also have higher clone-forming capability then CD34- cells. There is no difference about the clone-forming capability between CD44+ cells and CD44- cells.Conclusions:1、The expression of cancer stem cells markers of ABCG2、CD44、CD34 is vary in different NPC cell lines. 2、ABCG2+、CD34+ cells of 5-8F have higher clone-forming capability then the ABCG2-、CD34-cells. 3、There is no difference about the clone-forming capability between CD44+ cells and CD44- cells.