The Influences Of GM-CSF On Macrophages Subtypes In Lung Injury Of Hyperoxia-Exposed Newborn Rats

ObjectiveThe preliminary research of the influences of GM-CSF on classification of macrophages subtypes in lung injury of hyperoxia-exposed newborn rats.MethodsNinety-six newborn SD rats were randomized into four groups : air-exposed group, air-exposed + GM-CSF group, hyperoxia-exposed group and hyperoxia-exposed + GM-CSF group. The rats in high oxygen group were placed in a sealed plexiglas chamber maintaining O2 1evels above 95 % and those in normal control group were exposed to air. The rats in air-exposed+GM-CSF group and hyperoxia-exposed + GM-CSF group with subcutaneous injections of GM-CSF(9ug/kg). Statistical analysis was carried out on the newborn mice weight. Adopting the flow cell technique with different antigen of two kinds of macrophages for different periods of macrophages double positive screening. The value of lung damage score calculated with histology under light microscope.Results(1) Compared with air group, the weight decreased in hyperoxia-exposed group( P<0.05).(2) Compared with air group, the expression of M increased in hyperoxia-exposed group at each time point(P<0.05). Compared with hyperoxia-exposed group, the expression of M decreased at 7st day, 21stday(P<0.05).(3) Compared with air group, the expression of M1 increased in hyperoxia-exposed group at each time point(P<0.05). Compared withhyperoxia-exposed group, the expression of M1 decreased at 3st day, 7stday(P<0.05).(4) Compared with air group, the expression of M2 increased in hyperoxia-exposed group at 3st day, 7st day, 21 st day(P<0.05). Compared with hyperoxia-exposed group,the expression of M2 increased at 3st day, 7stday(P<0.05).ConclusionsThe promotion of GM-CSF on classification of macrophages subtypes in lung injury of hyperoxia-exposed newborn rats.