The Correlation Of FHIT And TSLC1 Gene Expression And HPV Infection In Cervical Cancer

Background and ObjectiveCervical cancer was a common gynecologic malignant tumor. Its incidence was only after breast cancer. The incidence of cervical cancer in developing countries was significantly higher than that of in the developed countries. In recent years, it also shows younger trend year by year. A large number of epidemiological data showed, high risk human papilloma virus (HR-HPV) persistent infection is the leading risk factors for the development of cervical cancer. However, in the HPV infected individuals, only a very small number of patients would develop into cervical cancer, and not all cervical cancer patients were infected by HPV. Cervical cancer is a variety of factors act on together in a plurality of links on the results. The Inactivation of the tumor suppressor gene played an important role in the pathogenesis and development of cervical cancer on gene level. Fragile Histidine Triad gene (FHIT) and tumor suppressor in lung cancer 1 (TSLC1) were the two candidate tumor suppressor gene discovered in recent years. The loss expression of these two kinds of gene had a related with the pathogenesis and development a variety of tumor.Though detecting HPV infection and the expression of FHIT gene and TSLCI gene in cervical cancer tissue, different levels cervical intraepithelial neoplasia (CIN) tissues and normal tissues, this research aimed to study the correlation between FHIT and TSLC1 gene expression and cervical cancer occurrence, the correlation between FHIT and TSLCI gene expression and HPV infection and provide the reference for the early diagnosis of cervical cancer.MethodsThe patients were recruited from Department of Obstetrics and Gynecology of the Liaocheng People’s hospital for outpatient or hospitalization from June 2012 to June 2013 with a total of 180 cases. The basic clinical data of the patients was collected. The tissue samples were achieved through the pathology of cervical biopsy or excision operation organization. The patients were divided into inflammatory group, CIN I group, CIN II group, CIN III group, cervical cancer group and normal group according to incidence. Total RNA in tissue samples were extracted by using the Trizol kit. The expression of FHIT gene and TSLC1 gene in tissue samples was determined by using RT-PCR. The HPV-DNA of the samples was detected by using HC2 gene hybridization capture instrument and HPV-DNA detection kit. The data was analyzed by the SPSS17.0 statistical software.Results1. The positive rate of HPV infection in cervical cancer group, CIN Ⅲ group, CIN Ⅱ group, CIN Ⅰ group, inflammatory group and normal group of sample were 91.43%,85%,60%,40%,5.71%and 0%, respectively. Compared with the control group, cervical cancer group,CINⅢ group,CINⅡ group,CINⅠ group, HPV infection rate increased,the difference was statistically significant (P<0.05)2. The positive rate of FHIT gene expression in cervical cancer group, CIN Ⅲ group, CIN Ⅱ group, CIN Ⅰ group, inflammatory group and normal group of sample were 5.71%、35.00%.56.00%、72.00%,100.00% and 100.00%, respectively. Compared with the control group, cervical cancer group,CINⅢ group,CINⅡ group,CINI group, FHIT expression was significantly lower, the differerce was statistically significant (P<0.05).3. The positive rate of TSLC1 gene expression in cervical cancer group, CIN Ⅲ group, CIN Ⅱ group, CIN Ⅰ group, inflammatory group and normal group of sample were 2.86%,30.00%,52.00%,68.00%,100.00% and 100.00%, respectively. Compared with the control group, cervical cancer group,CINⅢ group,CINII group,CINI group, TSLC1 expression was significantly lower, the differerce was statistically significant (P<0.05)4. Expression of FHTT gene and TSLC1 gene associated with the infection rate of HPV DNA, and the HPV-DNA infection rate of the patients with the loss expression of FHTT gene and TSLC1 gene was significantly higher than that with the normal expression of FHIT and TSLC1 gene.5. Using the expression of FHIT to diagnose the cervical cancer, the sensitivity was 85.62%, specificity was 82.3%. Using the expression of TSLC1 to diagnose the cervical cancer, the sensitivity was 88.52%, specificity was 83.4%. Using the combination of FHTT and TSLC1 to diagnose the cervical cancer, the sensitivity was 94.23%, specificity was 95.3%. It illustrated clinical value of the combination of FHIT and TSLC1 was higher than that of TSLC1 or FHIT single diagnostic value. The difference was statistically significant (P<0.05).Conclusion:Through the detection of the HPV infection, the FHIT and TSLCI gene expression of the 180 cases with different severity of cervical tissue, we could get the following conclusion:l.The HPV infection was existed in the different levels of CIN and cervical cancer. The detection rate of HPV in the patients with cervical cancer was higher than that with CIN HI.2. The loss expression of FHTT gene and TSLCI gene was existed in the different levels of CIN and cervical cancer. The higher the level of CIN was, the lower the detection rate of FHIT gene and TSLCI gene was. The detection rate of FHIT gene and TSLCI gene in cervical cancer patients was the lowest.3. The HPV infection rate of the patients with the loss expression of FHTT gene and TSLC1 gene was significantly higher than that with the normal expression of FHTT gene and TSLCI gene.4. Joint detection of the FHIT gene and TSLC1 gene can improve the accordance rate of clinical diagnostic of cervical cancer and provide new research ideas for clinical prevention and treatment of cervical cancer.