Research On The Consistency Check Of Different Methods For Detection Of EGFR Status In Non-small Cell Lung Cancer

Aim:Lung cancer is the most common cause of cancer-related deaths worldwide, most of the patients are non-small cell lung cancer (NSCLC). This study was designed to investigate the consistency of different methods for the detection of the epidermal growth factor receptor (EGFR) gene status in NSCLC patients and elucidate their reliability and clinical practicablity.Methods1.242 NSCLC cases were enrolled in our study. EGFR gene amplification was determined by the method of FISH.52. EGFR gene mutation was examined by fluorescent probe-based PCR and DNA sequencing methods.Results1.242 specimens of paraffin non-small cell lung cancer patients in 170 cases of male,female 72 cases.150 patients≧60 years,92 patients< 60 years.Organization credits type:99 cases of squamous cell carcinomas; gland scale cancer 6 cases; infiltrating adenocarcinoma adherent growth form 24 cases,infiltrating adenocarcinoma non adherent growth form 113 cases.2. EGFR gene amplification is present in 27.27% of patients with lung cancer. EGFR gene amplification was present in 27.27% of patients. EGFR mutation was present in 35.12% of patients by sequencing method, and 32.23% by PCR method. The status of EGFR amplification was significantly associated with that of EGFR mutation.3. EGFR amplification is independent of age, but is significantly higher in females and patients with adenocarcinoma (all P<0.05). Both amplification and mutation status were independent of age, but were significantly higher in females and patients with adenocarcinoma (all P<0.05).4. In 242 cases,66 cases of EGFR gene amplification; Sequencing method validation hitches in 66 cases exist in 50 cases of EGFR gene mutation, it has been verified by PCR-probe method EGFR mutations in 46 cases exist. By chi-square test, the P value was 0.031 and 0.031, respectively (P<0.05), statistically significant, EGFR amplification and mutations exist positive correlation, namely EGFR amplification and the result of the mutation has significant correlation.Conclusions:In NSCLC patients, over-expression of EGFR gene might be related to EGFR gene amplification and mutation. FISH method is a sensitive method for the detection of non-small cell lung cancer EGFR amplification. FISH method is expected to collaborate with EGFR mutation detection methods to guide the selection of EGFR individualized treatment programs and the assessment of prognosis.