The Mechanism Study Of Postmenopausal Osteoporosis In Rats MAPK Channel With Embedding Therapy

ObjectiveThis research on embedding therapy for postmenopausal osteoporosis in rats MAPK channel by observing estrogen levels, bone density, mitogen-activated protein kinase (MAPK), to explore the mechanism of postmenopausal osteoporosis disease (PMOP) in transduction pathway MAPK signaling by catgut embedding, to study the mechanism on prevention of osteoporosis by catgut embedding, as to guide clinical conduct catgut embedding therapy for osteoporosis treatment and provide a strong experimental basis, this can enrich the theory of acupuncture treatment of postmenopausal osteoporosis,to guide clinical practice.Methods60 female Sprague-Dawley rats were divided into some groups:named A, B, C, D, E, F groups, randomly divided into six equal groups,10 rats in each group. All experimental animal anesthesia by intraperitoneal injection, the other group except F group, ovariectomized group, F were exposed after ovarian suture, as sham operation group. After treatment for 4 months after the treatment model, group A:catgut embedding therapy, once every 5 days; B group, catgut embedding therapy, once every 10 days; group C catgut embedding therapy for 15 days once; group D diethylstilbestrol solution by gavage, every 5ml/kg, every 1 days; E and F group and normal saline, each 5ml/kg,1 days at a time. Treatment of all executed after 3 months. Detection of femoral bone mineral density; serum E2; mitogen activated protein kinase (MAP2K1) in the femur.Results1. the femur BMDCompared with E group, A group and D group rats BMD have significant difference (P< 0.05 respectively, P< 0.01) other group increased but not significantly (P> 0.05); compared with group F (sham operation group),A, B, C, D, E in rats BMD has decreased, and the difference of A and D was not significant (P> 0.05), the other group with significant difference; A, B, C group compared with the drug group, the difference was not significant (P > 0.05); the gradient catgut embedding treatment group A, B, C groups were compared with each other, no statistical significance in the the difference (P>0.05).2. the content of E2 in serumCompared with F group, E group rats serum E2 levels were significantly lower (P< 0.01); compared with E group, the level of serum E2 of rats in C group had no significant difference (P>0.05), elevated A, C, D in serum with different degrees of E2 (P< 0.01 or P< 0.05); treatment groups compared with the F, A, D group E2 in serum of rats were decreased but the difference was not significant (P> 0.05), B, C group E2 decreased significantly (P< 0.05); but compared with each two group between the treatment group A, B, C (P>0.05), no significant difference.3. the expression of MAP2K1 protein in bone tissue of femoralCompared with group F (sham operation group), MAP2K1 protein in bone tissue in group E was significantly reduced (P< 0.01); compared with group E, bone tissue MAP2K1 protein in A, B, C, D group rats were increased (P< 0.05 or P< 0.01); A, B, C group compared with drug group, the difference was not significant (P> 0.05); catgut embedding group data comparison, no significant difference (P>0.05).ConelusionCatgut embedding method improved the bone mineral density on rat model of postmenopausal osteoporosis,this method increased the content of serum estradiol level and have increased femur bone tissue protein MAP2K1, improved that embedding therapy achieve the treatment of postmenopausal osteoporosis purposes by impacting these indicators, the possible Mechanism of osteoporosis treatment of postmenopausal:1 embedding method have estrogenic effects on the hypothalamic-pituitary-gonadal axis have a moderating effect; 2 points embedding method by influencing MAP2K1 protein, thus affecting bone formation MAPK -related channel, which may be one of the main mechanisms of osteoporosis after menopause treatment of the Act.