Expression Of Heat Shock Protein27 In Injury Healing Of Human Gingival Fibroblast In Inflammatory Environment

Background and purpose:Peri-implantitis is a major cause of implant failure. Peri-implantitis, namely, inflammation, chronic progressive edge is considered an inflammatory reaction, which causes the function of supporting bone around implant implant failure due to absorption. Peri-implantitis s pathological changes mainly for gum tissue to grow on the surface of the body surface adhesion ability is low or even no adhesive ability, healthy tissue and bone loss of merger, and local IL-1 beta, LPS levels of inflammatory factors such as healthier place rise and rise with the pathological changes of trend. But Peri-implantitis research is at the stage of providing clinical diagnosis index by discovering and examining inflammation factor in peri-implant gingiva groove liquid. There is no report aboutits occurrence and development in the process of cellular pathways and key protein research.This study was aimed to investigate the expression of heat shock protein (Hsp27) in human gingival fibroblasts (HGFs) during injury induced by different concentrations of interleukin-1β、 LPS、CSE. The experimental’s focus is the observation of correlation between Hsp27 and gum tissue wound healing, which provides the experimental basis for the later research and revealing the role mechanism of Hsp27 in Peri-implantitis.Methods:(1) The primary cultured of human gingival fibroblast;(2) Immunohistochemical identification of cell sources;(3) HGFs were treated with serially diluted IL-1β、LPS and CSE;(4) Would healing assay was used to determine the migration of HGFs;(5) Western blot was used to determine the expression of Hsp27 and Hsp70 and Hsp90 in HGF in vitro.Results:(1) Immunohistochemical experiment shows primary culture cells are negative for anti-keratin antibody and positive for resistance waveform silk protein antibody. Cytoplasm is dyed buffy and the nucleuses are dyed blue. The results prove that cells are gingival fibroblasts source, in orther words, cells are mesoderm organization sources not epithelium.(2) After intervention of IL-1 beta, LPS and CSE, migration ability of HGFs significantly reduced following the increased concentration of IL-1 beta, LPS and CSE stimulation.(3) Expression of Hsp27 was positive in a concentration-dependent manner, the expression of Hsp70 and Hsp90 has no obvious change.(4) Expression of Hsp27 was positive in HGFs intervened by LPS in a concentration-dependent manner. Wound healing assay showed that HGFs has lower migration capability with higher LPS concentration and with negatively Hsp27 expression quantity.(5) Expression of Hsp27 was positive in HGFs intervened by LPS in a concentration-dependent manner. Wound healing assay showed that HGFs has lower migration capability with higher CSE concentration and with negatively Hsp27 expression quantity.Conclusions:Concentration of Hsp27 became higher than usual in IL-1β、LPS、CSE induced injury HGFs and positively related to the cell migration. That suggests Hsp27 plays an important role during the occurrence and development of wound healing, and the underlying mechanisms remain to be further investigated.