Aflatoxin B1-induced Oxidative Stress And Nutrional Intervention In Hepatocyte L-02

Mycotoxins are closely related to human life, and contaminate approximately 25% of the world’s foodstuff according to Food and Agriculture Organization of United Nations (FAO). They were involved into the food chain as secondary metabolites of fungus through contaminated grain and feedstuff. Therefore, the damage and nutritional intervention about mycotoxins are highly concerned. This experiment was conducted through prying oxidative damage effect of Aflation B1 (AFB1) on human liver cells as the research object and exploring effects of nutritional intervention of such nutrient elements as chlorogenic acid (CGA) and quercetin (QU), which might prospectively provide reference on easing the AFB1 damage to human health.The research methods and results are as follows:1) Overall, survival rate of L-02 cell was inversely proportional to concentration of AFB1 despite being promoted by little dosage. AFB1 could reduce the concentration of protein and affect the enzyme activities of Glutathione (GR), and the protein concentration gradually reduced with the increase of AFB1 concentration in the L-02 cell. Glutathione reductase (GR) and Glutathione Peroxidase (GPx) content increased significantly when the cells were stimulated by small amount of AFB1. With the increase of AFBl concentration, the activities of both enzymes gradually were inhibited. Experimental concentration of AFB1 had no effect on Glutathione S-transferase (GST) activity. When AFB1 concentration was low (less than 125μm), Glutataione (GSH) content was directly proportional to concentration of AFB1. When AFB1 concentration was too high, the GSH content gradually reduced.2) CGA had promoting effect on the L-02 cell proliferation, and protein content increased with the increase of CGA concentration. CGA improved the GR and GPx activity of L-02 cell, but had little effect on the GST vitality in the L-02 cells. When L-02 cells are treated by AFB1, CGA could improve the vitality of GST in the L-02 cells. The low concentration of CGA enhanced the GSH content in the L-02 cells, and when concentration of CGA was 40g/ml, higher GSH content was received. The results showed that intracellular oxidative damage was alleviated.3) When the concentration of QU was lower than 160μg/ml, QU had promoting effect on the L-02 cell proliferation. And it had toxic effect on cells when the concentration reached 320μg/ml. After cells were treated by AFB1, QU improved the content of intracellular proteins, and changed activities of the correlated anti-oxidase enzyme. QU played an important role in promoting the vitality of GR, GPx and GST in L-02 cells. QU could promote the formation of intracellular GSH, and when the concentration of QU reached 40μg/ml, higher GSH content was received in L-02 cells. Therefore, QU had great effect of intervention.