| ObjectiveDiscussion intervention Jianpibushenjiedu method monkey model of AIDS.MethodsWith Chinese rhesus monkeys eight monkeys were randomly divided into 2 groups:group of four Chinese medicine, western medicine group of four. All the monkeys had by intravenous SIVmac239 AIDS model is set up. Chinese traditional medicine group was given traditional Chinese medicine (TCM) suspension to fill the stomach; The western medicine group was given PMPA precursor (tenofovir) subcutaneous injection. Respectively before the dosage, dosing in January, for over two months, stop drug withdrawal in January, when peripheral blood plasma viral load test, CD4+T lymphocytes and CD8+T lymphocytes, indices such as serum IL-21; Before delivery, delivery end, stopped two months to collect superficial lymph node detection of CD4 molecules and IL-21 indicators; At the end of two months after stopping drug experiment acquisition such as gastrointestinal organization detect CD4 molecules and IL-21 indicators, and the data statistics processing.ResultsTwo groups of monkeys in the peripheral blood plasma viral load besides T2 time points, the rest of the time between the two groups associated with plasma viral load is not big, has less variation in the group. T2 time for 2 months after treatment, western medicine group after antiviral treatment, there is a monkey viral load decreases obviously, so mean the western medicine group was obviously lower, but each monkey fall in varying degrees, in the group a little rise instead, large variation in the group, and the standard deviation is big also. At various time points, the differences between the two groups, were not statistically significant.A monkey in Chinese traditional medicine group in the late death, five basic stable overall level of plasma viral load testing time points. Specific individual monkeys, in which 542 monkeys presented gently falling, and it’s viral load has been the highest, and the only performance continued to decline. The other two in baseline small-scope fluctuation, stable performance. But 558 monkeys appear relatively large fluctuations, first presents the obvious drop, then the plasma viral load there is rising sharply, began more than the baseline value, the final death. The plasma viral load testing time points, had no statistical significance.In the western medicine group, a total of four monkeys there are two monkeys in death,540 of them have died in the PT-T1 time period,537 died in T2 time points. Five testing time points in the process of experiment, the overall level of plasma viral load has some changes, especially in the two months after the treatment, namely T2 time points, to minimize the overall level of plasma viral load, a drop of about 1/2. Specific view mainly is 532 and 535 the monkey plasma viral load levels on the decline, especially 535 monkeys, dropped to below the detection level directly, make this a significant decline in plasma viral load directly, but after stop in western medicine,532 and 535 monkey plasma viral load level a sharp rebound, directly back to the level before treatment. Also show differences in the group, including 537 monkey plasma viral load did not fall but rise to a certain extent, viral load levels has been at the highest level, and death.532 and 535 monkeys showed the sensitivity of the antiviral treatment of western medicine, and to survive to the end of the experiment, to see the monkey from the result of the real individual evident, similar to the human condition, different sensitivity to the same antiretroviral drugs. This group, because of the large variation in the group the plasma viral load testing time points, both showed no statistical significance.Two groups of monkeys in each testing point of peripheral blood CD4+ T lymphocytes percentage in addition to the point in time before treatment, other each point in time, the traditional Chinese medicine group of monkeys mean (peripheral blood CD4+T lymphocyte percentage is higher than western medicine group, over time, the differences between the more obvious. But two groups, each group within a larger difference, the value of the standard deviation is relatively high. But two groups of each point in the peripheral blood CD4+T lymphocyte percentage difference comparison, there were no statistical significance.Two groups of monkeys peripheral blood CD4+T lymphocyte percentage comparison is roughly the same change trend, generally characterized by a rise, after again downward trend; But two curve values are very different, in front of the starting point for treatment, Chinese medicine group is lower than the western medicine group, the two curves intersect, after the treatment of subsequent each point in time, are characterized by traditional Chinese medicine than western medicine group. Group in the experiment, the traditional Chinese medicine (TCM) weeks of blood CD4+T lymphocyte percentage value basic hold this before the treatment, while the western medicine group fell sharply. Two groups of monkeys outside at each time point of blood CD4+T lymphocyte percentage value, by the repeated measurement data analysis of variance, time effect, the effect between groups, the interaction effect of no statistical significance.Chinese traditional medicine group at various time points of CD4+T lymphocyte percentage each monkey change within the group is not very consistent, the T1 time increased significantly, mainly due to the 558 monkeys CD4+T lymphocyte percentage rise sharply lift, and 545 monkeys also appeared a slight decline, other two monkeys showed up. In T2 time points, both in a relatively low rise, both in a relatively high value was significantly decreased, the overall showed a downward trend, from five time point of view, in addition to 558 monkey swings the most obvious and death, the rest of the monkey or a relatively stable and has survived to the end of the experiment. But each time point, no statistical significance.Western medicine group of monkeys at various time points of peripheral blood CD4+T lymphocyte percentage point rise slightly in the T1 time, later time points are continue to fall, observe dropped to its lowest after the drug was stopped, is lower than before treatment. One of the 540 and 537 the monkey died in the experimental process; 540 of them occurred in the treatment of early death, but the cause of death in combination with other indicators to further analysis, and 537 deaths occurred at the end of the treatment with a monkey, but the CD4+T lymphocyte percentage is not low, the opposite is the highest in the group. Each point in time, no statistical significance.Two groups of monkeys in each testing point of peripheral blood CD4+ T lymphocyte count each point in time, the Chinese medicine group were higher than western medicine group, and after treatment intervention, the advantage of traditional Chinese medicine group is more obvious. But differences in the Chinese traditional medicine group, and wide standard deviation values. The western medicine group is relatively small. At all time points, two groups of monkeys peripheral blood CD4+T lymphocyte count difference comparison, there were no statistical significance.Two groups of monkeys in each testing point of peripheral blood CD4+ T lymphocyte count change trend of Chinese traditional medicine group of CD4+T cell count value in the treatment of continuous rise, appeared in the process of sampling period at the end of the treatment has to rise again after the fall, at the end of the experiment, this group of peripheral blood CD4+T lymphocyte count is obvious compared to that before treatment, the rising trend of fluctuations in general; And a month in the treatment of western medicine group rose slightly from time to tome, the late continues to decline, have a small recovery in the end of the experiment, at the end of the experiment, a slight decline than before treatment, the overall assumes the declining trend of fluctuations. Two groups of monkeys peripheral blood CD4+T lymphocyte count in five different point in time, by the repeated measurement data analysis of variance, time effect was statistically significant (P<0.05), between group effect, the interaction effect, no statistical significance.Chinese traditional medicine group each monkey at various time points of CD4+T lymphocyte count in T1 and T2 time gradually rise, the timing of the subsequent decline, but are higher than before treatment, among them 558 monkeys CD4+T lymphocyte count has been at the lowest, and death; No. 545, the monkey is (monkeys are) the whole experiment process of the best monkey, actually CD4+T lymphocyte count continued at a high level until the end of the experiment. The differences between each point in time, all showed no statistical significance.Group of western medicine,540 monkeys of CD4+T lymphocyte count the start at a minimum, and in the treatment of early death, and no.537 monkeys in the process of treatment, the CD4+T lymphocyte count has increased dramatically, and has been high, but at the end of the treatment and death occurred. But monkeys with 540,535 monkeys, CD4+T lymphocyte counts in the process of the whole experiment has been maintained at low levels, but to survive to the end of the experiment; In early 532 monkeys changes with the change of the number 537 is similar to that of treatment is 2 months difference is bigger,532 monkeys dropped sharply in the CD4+T lymphocyte count, even lower than before treatment, but also to survive to the end of the experiment. From the point of this study, a single view of CD4+T cell count index may not be enough to judge prognosis, is discussed in combination with other indicators to do analysis on; CD4+T lymphocyte count at each time point difference comparison, there were no statistical significance.Two groups of monkeys in each testing point of peripheral blood CD8+ T lymphocytes percentage discontinuation in addition to the observation point in time of a month, Chinese traditional medicine group of CD8+T lymphocytes percentage higher than west group, other each point in time, the Chinese medicine group were lower than western medicine group, and the difference between maintaining stable. But later the variation in the group of western medicine is bigger, the standard deviation value is larger. At each time point, the traditional Chinese medicine and western medicine group of peripheral blood CD8+T lymphocytes percentage difference comparison, were not statistically significant.In each testing point of peripheral blood CD8+T lymphocytes percentage overall trend is close to the two groups, the overall higher than in traditional Chinese medicine group and western medicine group. Western medicine group spans a relatively large changes in peak peak, and is low in the low. At the end of the experiment, two groups of CD8+T lymphocytes percentage were increased compared to that before treatment. In different time, the traditional Chinese medicine and western medicine group of CD8+ T lymphocytes percentage difference comparison, through the analysis of variance of repeated measurement data, time effect was statistically significant, p<0.05, effect and exchange effect between groups had no statistical significance.Chinese traditional medicine group,558 monkeys CD8+T lymphocytes percentage in the process of treatment has been rising, and reached its highest, but there was death, but 542 monkeys also rose in the treatment, but has been in a relatively low status, and increase amplitude is small, but to survive to the end of the experiment, the other two monkeys is successively elevator fluctuation change, smooth alive at the end of the experiment. The percentage of CD8+T lymphocytes in time difference, there is no statistical significance.Western medicine group each monkey all time points before and after the treatment of CD8+T lymphocytes percentage in T2 to S1 point appear bigger difference,532 monkeys decreased obviously, and 535 continue to rise. Including 540 monkeys in the starting point in a relatively low, and the death,537 monkeys of CD8+T lymphocytes percentage rise all the way, also in T2 time points after death occurred. This group of each time point of CD8+T lymphocytes percentage difference comparison, there were no statistical significance.Two groups of monkeys at various time points of CD8+T lymphocyte count results at each time point, Chinese medicine group than in the west medicine is high, and the variation in the group of Chinese traditional medicine is bigger, the standard deviation value comparison group of western medicine. Each point in time, the differences between two groups of comparison, there were no statistical significance.Two groups at various time points of CD8+T lymphocyte count results change tendency were showed increased trend of fluctuations in the treatment of a month is groups gain is greater than the traditional Chinese medicine, western medicine basic close to the level of the two groups, but two months after treatment, the rise in Chinese traditional medicine group, and basically reached the same level of the western medicine group after discontinuation of two sets of changes, at the end of the experiment, two groups of CD8+T lymphocyte count were higher than before treatment. The difference between two groups at various time points, by analysis of variance of repeated measurement data, time effect, effect and interaction effect between groups had no statistical significance.Chinese traditional medicine group at various time points of CD8+T lymphocyte count results change is mainly caused by 545 monkeys fluctuations, number during the period of traditional Chinese medicine treatment the monkey CD8+T lymphocyte count is an obvious rising trend, but also has a higher peak after the drug was stopped, so can’t determine its changes related to traditional Chinese medicine treatment, and another 558 monkeys CD8+T lymphocyte count has been very low, and death after T1 time points. The other two number of monkeys CD8+T lymphocyte count change is not big, each point in time are very stable. From a statistical perspective, the comparison of each point in time, is meaningless.Western medicine group at various time points of CD8+T lymphocyte count results in PT point in time, at a minimum,540 deaths occurred shortly after the start of treatment. No other monkeys CD8+T lymphocyte count on T1 time points are on the rise, and T2 time point were flat or falling, and 537 monkeys have continued to rise, peak, and death. Live monkey in S1 time points of CD8+T lymphocyte count were hit bottom, after the rebound in S2 time; The comparison between each time, there is no statistical significance.Two groups at various time points of CD4+T lymphocytes and CD8+T lymphocyte ratio before the treatment, two groups of CD4+T cells and CD8 +T lymphocyte ratio close to, and differentiation in after treatment, follow-up on all the time, Chinese traditional medicine group of CD4+T lymphocytes and CD8+T lymphocyte ratio is western medicine group. At each time point, the difference between Chinese medicine and western medicine group, there were no statistical significance.Two groups at various time points of CD4+T lymphocytes and CD8+T lymphocyte ratio change trend are obviously different, Chinese traditional medicine group increased obviously after treatment, treatment appeared when the ratio is greater than 1 a month, back later to a certain extent, but the basic flat, change and stability. Western medicine group presents the declining trend, at the end of the experiment, has dropped to low point, significantly lower than the level before treatment. But the difference between two groups of each point in time, by analysis of variance of repeated measurement data, time effect, effect and interaction effect between groups had no statistical significance.Chinese traditional medicine group in which 558 monkeys CD4+T lymphocytes and CD8+T lymphocyte ratio to rise all the way, monkeys are higher than others, and death occurred. That is too much that is a good thing, may indicate an immune activation strengthening state, triggering an immune storm and die. Other monkeys is stable within a certain range, to survive to the end of the experiment.Western medicine group in which 540 monkeys of CD4+T lymphocytes and CD8+T lymphocyte ratio is relatively low, and occur in the treatment of early death, monkeys appeared after a sharp rise in 537 have fallen sharply, there have also been killed. The remaining two monkeys, fluctuated state of decline, and always to survive to the end of the experiment. At the end of the experiment, two monkeys of survival of CD4+T lymphocytes and CD8 +T lymphocyte ratio is lower than before treatment.Superficial lymph nodes in the two groups at various time points CD4 molecules immunoblot grey value at each point in time, traditional Chinese medicine (TCM) was slightly higher than western medicine group, the difference between groups, were not statistically significant. Both groups showed a trend of decline. But after the first decline in Chinese traditional medicine group, flat, has been higher than western medicine group level, and the western medicine group decreased more obvious. But the differences between the two groups to compare, after repeated measurement data analysis of variance, time effect, effect and interaction effect between groups had no statistical significance.Two groups of stomach tissue CD4 molecules immunoblot grey value results western medicine group slightly higher than that of traditional Chinese medicine group, two groups of group differences in close, and the difference between the two groups to compare, no statistical significance.Two groups of duodenal tissue CD4 molecules immunoblot grey value results traditional Chinese medicine than western medicine group, two groups of difference is not significant.Two groups of the ileum tissue CD4 molecules immunoblot grey value results traditional Chinese medicine group is higher than western medicine group, Chinese medicine and western medicine group, the difference was statistically significant (p<0.05).Two groups of colon tissue CD4 molecules immunoblot grey value results traditional Chinese medicine group was a little higher than western medicine group, the differences between the two groups, no statistical significance.Two groups of rectal tissue CD4 molecules immunoblot grey value higher in Chinese medicine than western medicine group. Traditional Chinese medicine and western medicine group differences, with statistical significance (p<0.05).Two groups of mesenteric lymph nodes CD4 molecules immunoblot grey value higher in Chinese medicine than western medicine group. Traditional Chinese medicine and western medicine group, the difference was statistically significant (p<0.05).Two groups at various time points of serum interleukin 21 results before the treatment, western medicine group of serum interleukin 21 was slightly higher than that of traditional Chinese medicine group, the differences between the two groups, there is no statistical significance. But the value of traditional Chinese medicine group was obviously increased after treatment, in the western medicine group only slightly increased, clear difference in the two groups, with statistical significance (p<0.05); Stopped to observe two months later, the two groups of serum interleukin 21 have retreated, the back of traditional Chinese medicine group is also very obvious, but still slightly higher than that of western medicine group, higher than before treatment; While the western medicine group is lower than before the treatment, but the differences between the two groups is not statistically significant.Two groups at various time points trend of serum interleukin 21 result difference is obvious. Chinese medicine group obviously increased significantly after the treatment, but after the drug withdrawal and apparent way, Chinese medicine treatment effect is obvious, but the present is the short-term effect, the withdrawal has fallen significantly, the rise of the western medicine group was only slightly, subsequent continue to fall, has been in a low level. Differences between the two groups to compare, after repeated measurement data analysis of variance, time effect, effect and interaction effect between groups have statistical significance (p<0.05).Chinese traditional medicine group before the time of serum interleukin 21 treatment discontinuation and variation in observation group were smaller, and after the treatment, although each monkey serum interleukin 21 are rising, but the rising rate is not very consistent between, there are some variation in the group. Diff between this all the time, was statistically significant (p<0.05)Each time point of the western medicine group of serum interleukin 21 result is small variation within the group, three time difference between the comparison, no statistical significance.Superficial lymph nodes in the two groups at various time points interleukin 21 results at each time point, Chinese traditional medicine than western medicine group is a bit high, but the differences between the two groups of comparisons, no statistical significance.Superficial lymph nodes in the two groups at various time points interleukin 21 result is consistent trends, tend to decline, at the end of the experiment, two groups of superficial lymph nodes interleukin 21 value is lower than before treatment, compare the difference between the two groups, the variance analysis of repeated measurement data, time effect, effect and interaction effect between groups had no statistical significance.Chinese traditional medicine group at various time points of superficial lymph nodes interleukin 21 results which have been relatively low value is no.558 monkeys, death, other variation is relatively small, at a high value of monkeys to survive to the end. The comparison of difference groups all the time, no statistical significance.The western medicine group at various time points of superficial lymph nodes interleukin 21 results in the treatment of before and after treatment for 2 months time, under the mean lower monkey all death happened during the testing process; Our group at various time points difference comparison, there were no statistical significance.Traditional Chinese medicine and western medicine group of stomach and duodenum, ileum and colon, rectum, and mesenteric lymph node groups interleukin 21 value comparison, traditional Chinese medicine than western medicine group, two groups of group differences are relatively large. The difference between two groups of comparison, there were no statistical significance.ConclusionSpleen and kidney detoxification method of traditional Chinese medicine compound monkey on HIV/AIDS model has certain treatment function. To improve HIV/AIDS model monkey peripheral blood CD4+T lymphocyte number, number of CD8+T lymphocytes and the ratio of both have certain effect; To improve HIV/AIDS model monkey peripheral blood levels of IL-21 have used; Monkey on HIV/AIDS model has a role in restoring gastrointestinal immune function. |
PDF Full Text Request