Study On The Chemical Constituents Of The Stem Of Cymbaria Dahurica L.

Cymbaria dahurica L., is a member of Cymbaria which belongs the family of Scrophulariaceae. It is used as good mongolian medicine which is extensively distributed in the xeropoium including Inner Mongolia, Hebei Province, Northeast of China. The root, stem, leaves and flowers of Cymbaria dahurica L. have been used as medicine for the treatment of expelling wind-damp and skin wound. It is also a good diuretic. It was previously reported that the chemical constituents of Cymbaria dahurica L. contain flavanoids, iridoids, carbohydrates, and cinnamic acid derivatives, some of which are all benefical to human health, so that these chemical constituents have been increasingly concerned in the area of healthcare and biochemistry. Until now the chemical constituents was reported in only two papers, in order to lay the foundation for developing and making the best of the mongolian medicine, the chemical constituents of the stem of Cymbaria dahurica L. has been investigated.In the thesis, first, the dried and comminuted stems of Cymbaria dahurica L. were extracted by 95% ethanol at 60℃. The extraction was mixed with kieselguhr by 1:1, then extracted successively by petroleum ether, ethyl acetate and n-butanol by Soxhlet extraction. Petroleum ether and ethyl acetate extracts were purified by applying column chromatography, TLC, recrystallization and centrifugation techniques. Four compounds have been obtained. The structures of four compounds have been identified by elemental analysis, MS, UV-VIS, IR,’H-NMR,13C-NMR, DEPT, HSQC, 1H-1H COSY and HMBC. Mannitol,2-octenoic isobutyl ester, isobutyl(3-heptacosyloxy)acrylate and daucosterol were isolated and identified from the stem of Cymbaria dahurica L. for the first time. Fifty more compounds were isolated and identified from the stem of Cymbaria dahurica L. by GC-MS for the first time, such as vitaminE, benzothiazole, limonene, β-ionone, (E,E)-farnesol, dihydroactinolide, perhydrofarnesyl acetone.