The Effects Of Conditioned Medium Local Transplantation In Repair Of Rat With Spinal Cord Injury

Objective To investigate the promoting effects of bone marrow mesenchymal stem cells conditioned medium(BMSC-CM) local transplantation on function recovery of rat spinal cord injuries.Methods The spinal cord of adult rats was injured with modified Allen method, and the rats were randomly divided into 2 groups. The rats in the control group were subjected to the microinjection of saline solution(10 μl) into the SCI site after SCI, and those in the experimental group were injected with the concentrated conditioned medium(10 μl) derived from the passage 3 BMSCs. The inclined plane test and Basso,Beattie and Bresnahan locomotor rating scale(BBB scale) were used to observe the recovery of rats’ behavioral function after the transplantation. Hematoxylin-eosin(HE)staining to reflect the change of cavity volume and angiogenesis at the lesion epicenter.Results BMSC-CM treated animals show better BBB scores and inclined plane scores than control animals, and this difference was statistically significant(P<0.05).BMSC-CM treated group shows reduced cavity volume and the blood vessels exhibited larger diameters compared to control group.Conclusion BMSC-CM can promote the recovery of neurological function in spinal cord injury, reduce cavity volume, and improve blood vessel growth.