Effect Of Treg /TH17cells In The Peripheral Blood Of Patients With Mycosis Fungoides On The Therapeutic Response

Mycosis Fungoides(MF) represent a paradigm for the heterogeneity and the dynamic variability of neoplastic disorders resulting in the accumulation of clonal lymphocytes in the skin. The severity of MF progress slowly and late can be developed into the tumor.It also can involved lymph and visceral. most MF(especially early stage) can be treated with non- chemical drugs,. As one of the classical therapeutic options, Ultraviolet irradiation and Interferon Injection can be used for early stage MF. Previously, We found that regulatory T cell(Treg) / Th17 imbalance exists in patients with MF and may effect the therapeutic response of MF. Our studies is to compare the therapeutic effect between narrow band-utraviolet B(NB-UVB) and NB-UVB combined with interferonα-2b in the treatment of patients with early stage mycosis fungoides(MF), and explore the correlation between Treg and Th17 cells in the peripheral blood of MF patients and clinical effects.Objective To compare the therapeutic effect between narrow band-utraviolet B(NB-UVB) and NB-UVB combined with interferonα-2b in the treatment of patients with early stage mycosis fungoides(MF), and explore the correlation between Treg and Th17 cells in the peripheral blood of MF patients and clinical effects.Methods 33 cases of MF patients in stage A-ⅡA were randomly divided into radiation group and combined group, radiation group was given NB-UVB irradiation alone andcombined group were given NB-UVB combined with interferonα-2b intramuscular injection, respectively. The percentage of Treg and Th17 cells in peripheral blood was determined by flow cytometry before and after treatment. 10 cases of health volunteers were selected as the control group.Results The average treatment time was 9 months. The response rates were 60.00% and 94.44% in radiation group and combined group, respectively, and the curative effects of the combined treatment was significantly better than the single treatment(P<0.05). The changes of Treg and Th17 cells in the peripheral blood before and after the treatment, The percentage of Treg and Th17 cells in peripheral blood before the treatment was significantly higher than that in the control group(P<0.05), but there was no significant difference between radiation group and combined group(P>0.05). The percentage of Treg and Th17 cells in peripheral blood of MF patients were obviously decreased after treatment, but still higher than the control(P<0.05). The amplitude reduction of Treg and Th17 cells in the peripheral blood of combined group was significantly greater than that of radiation group(P<0.05). Moreover, the curative effect was positively correlated with amplitude reduction of Treg /Th17 cells in the peripheral blood of MF patients(P<0.05). Treg cells of 7 cases of MF patients with no responding to the treatment significantly decreased, too.(P<0.05), while Th17 cells did not significantly decrease(P>0.05).Conclusion The curative effect of NB-UVB irradiation combined with INF- α-2b intramuscular injection was significantly better than that of only using NB-UVB in treatment of MF, and this was correlated with the decreasing amplitude of Treg and Th17 cells in the peripheral blood.