Study On The Expression Of Albumin, S100B And GFAP In Brain Of Rats With Epileptic Seizure Induced By Kainic Acid

Part Ⅰ The Expression of Albumin,S100B of Cerebrospinal Fluid in Kainic Acid Incuded Epileptic Rats[OBJECTIVE] To investigate the content of albumin and S100B in cerebrospinal fluid at different time points in kainic acidinduced epileptic rats.[METHOD] Seventy-five healthymale SpragueDawley rats were divided into five groups randomly, the normal control group (6h) and the epilepsy model groups(6h、24h、24h and 72h). The epilepsy modelrats were induced via intracerebroventricular injection (i.c.v)of kainic acid(KA),and collected cerebrospinal fluid(CSF) from the foramen magnum at time points of 6h,24h,3d,7d.Used enzyme linked immunosorbent assay(ELISA) method to detect the expressions of albumin and S100B in cerebrospinal fluid.The control group rats were intracerebroventricular injection of physiological saline.[RESULT] (1)The expression of albumin in CSF increased imme diately after epileptic seizure, the highest value arised in the 6h model gr oup (188.75±42.87μig/ml), then declined respectively at other model grou ps,the concentration of albumin in CSF of all model groups(153.19±34.80μg /ml,150.42±39.71μg/ml,138.52±31.16μg/ml) were significantely higher than t hose of the control group (127.45±28.32μg/ml) (p=0.007). (2)The expressi ons of S100B in CSF after epileptic epileptic seizure, The highest value a ppeared in the 3d model group (68.50±5.63 pg/ml),the concentration of S100B in the CSF of all model groups (36.60±2.77pg/ml,49.08±2.79 pg/m 1,41.37±3.14 pg/ml) were significantely higher than those of the control gr oup (31.60±3.55 pg/ml) (p<0.001).[CONCLUSION] The expressions of albumin and S100B increased after epileptic seizure in rats.Part Ⅱ The Expression of Albumin,S100B and GFAP in Brain Tissue and Structure Change of Hippocampus CA3 Regionby HE stainof Kainic Acid Induced Epileptic Rats at Different Time Points[OBJECTIVE] To investigate the temporal and spatial variation of brain tissue expression change of albumin,S100B and GFAP ofkainic acid induced epileptic rats, to exlpore their roles in the pathogenesis of epilepsy.[METHOD] Seventy-five healthy maleSpragueDawley rats were divided into five groups randomly, the normal control group (6h) and the model groups(6h、24h、24h and 72h). The rats of model groups were injected KA into the right lateral ventricle,and were executed at time points of 6h,24h,3d, 7d.To detect the expression of albumin,S100B and GFAP in hippocampus and albumin in the lateral ventricle and cortex by immunohistochemistry method and use HE stain method to observe the structural and cell morphological changes of hippocampus CA3 region.[RESULT] (1)The expressions of albumin in the lateral ventricle, S100B and GFAP in hippocampus increased after epileptic seizure, the highest value appeared respectively in the 6h model group,3d model group and 7d model group,the concentrations of albumin,S100B andGFAP of all model groups were significantely higher than those of the control group (albumin:p=0.007,S100B and GFAP:p<0.001).Albumin were also visible in the primary somatosensory cortex and the hippocampus CA3 region in individual rats. (2) After epileptic seizure, cells in the hippocampal CA3 region disorganized with morphology changed, dwindled in numbers and cell nucleus anachromasis,and these changes were aggravated with time.[CONCLUSION] (1) Epileptic seizure can cause the expression of albumin in the ependyma of lateral ventricle, S100B and GFAP in hippocampus increased,and albumin deposited in the primary somatosensory cortex and the hippocampus CA3 region; (2)The epileptic seizures can result in the degeneration and necrosis of neurons in the CA3 area of the hippocampus, and the number of neurons decreased.