| Objective To investigate the differentiation of human olfactory mucosa mesenchymal stem cells into retinal cells in vitro.Methods Through the olfactory mucosa adherent method, olfactory mucosa were isolated, cultured and indentified in vitro between mesenchymal stem cells.Using flow cytometry instrument analysis of the cell surface markers, in vitro induced to cells expressing photoreceptor-specific marker rhodopsin, and the identification of immunofluorescence method and Western blot analysis.Result OM-MSCs from human were spindle cell-based, showing radial colony arrangement. OM-MSCs were negative for CD34ã€CD45 and CD105, but positive for CD73 and CD90.Following induction, photoreceptor specific marker rhodopsim produced a strong reaction in cellsConclusion It is concluded that OM-MSCs can be cultured and expanded in vitro, which possess biological characteristics ofmesenchymal stem cells,and have the ability to be induced into retinal cells. |
