Correlation Study Between The Expression Of E-cadherin And β-cantenin With Infiltration In Invasive Ductal Carcinoma

Objective: The aim of our study is to investigate the expressio-ns of E-cadherin and β-catenin in invasive ductal carcinomas(IDC) and evaluate the correlation of these expressions with their invasion. 1. Methods : Immunostaining of E-cad and β-catenin was performed from paraffin specimens of 129 IDC,20 ductal carcinoma in situ with microinvasion(DCIS-MI),20 ductal carcinoma in situ(DICS) and 20 fibroadenoma. 2. Evalua-ting the expressions of ER, PR, HER-2, Ki-67 in IDC and repeating the determination of pathological sections. Then according to the expression of each index for grouping. Dividing IDC into four types: Luminal A, Luminal B, the expression of Her-2 and triple Negative with the St.Gellen classification. To stag group with the pathological grade of WHO(WHO), the maximum diameter of tumor(T),axillary lymph node metastasis(LN), distant metastasis(M), pTNM on the basis of America Joint Committee on cancer(AJCC). 4 Grouping all paraffin specimens for 4 types:IDC, DCIS-MI, DCIS and fibroadenoma. At last, evaluate the correlation of the expressions of E-cad and β-catenin in each group. Results: 1.There are different degrees of loss of the expression of E-cad expression(+~++) in IDC,DCIS-MI, DCIS and fibroad-enoma, even though in all tumor cell membrane are expressed, each type the normal expression(+ + +) rates were 41.9%, 40%, 80%, 90%.The relationship between the abnormal expression of E-cad with the patient’s age, number of LN and M was not significant(P > 0.05) in IDC. Having significant difference(P < 0.05) in WHO, tumor size, tumor tissue pathological staging, ER, PR, Ki-67 and molecular subtypes. The differences of the abnormal expression of E-cad in IDC, DCIS-MI and DCIS were significant(χ2=19.450,P=0.000). The abnormal expression of E-cad in DCIS was obviously lower than that of IDC and DCIS-MI, and the IDC and DCIS-MI approaches. The abnormal expression of E-cad in the presence of statistically significant differences in IDC and fibroad-enoma(P < 0.05), the abnormal expression rate of IDC was significantly higher than that infibroadenoma(P < 0.05).2. β-catenin were expressed in the tumor cell membrane and cytoplasm of IDC,DCIS-MI, DCIS and fibroadenoma. The loss of membrane experssion degrees were different in IDC, DCIS-MI, DCIS and fibroadenoma. Each type’s normal expression(+ + +) rates was16.3%, 35%, 60%, 70%. There were different levels of abnormal staining cytoplasm in IDC, DCIS-MI, DCIS and fibroadenoma. There were no difference between the abnormal expressionof β-catenin with age, tumor size, ER, PR, HER-2 and tumor size in IDC(P>0.05). With WHO grading, LN, M, pTNM staging, molecular typing and Ki-67 were significant difference(P < 0.05).The rate of cytoplasm and nucleus of the abnormol dyeing, HER-2 > triple negative > LuminalB > Luminal A, LuminalB in HER-2 was higher than that of non expression.The differences of the abnormal expression of β-catenin in IDC, DCIS-MI and DCIS were significant(χ2=23.694,P= 0.000). The expression intensity of β-catenin in each type : DCIS > DCIS-MI > IDC. The abnormal expression of β-catenin in the presence of statistically significant differences in IDC and fibroadenoma(P < 0.05), the abnormal expression rate of IDC was significantly higher than that infibroadenoma(P < 0.05). 3.The relationship between the abnormal expression of E-cad and β-catenin in the cell membrane of IDC has a significant correlation(r=0.324, P =0.034).The relationship between the abnormal expression of E-cad in membrane and β-catenin abnormal expressing in cytoplasm of IDC has a significant correlation(r=0.330,P =0.031). Conclusions: The relationship between the abnormal expression of E-cad with WHO,tumor size, pTNM, ER, PR, Ki-67 and molecular subtypes was significant difference(P < 0.05) in IDC.①The less differentiation, the more abnormal expression of E-cad was;②When the tumor is larger than 2cm the expression of E-cad was obvious worse. ③The negative of ER,PR was worse than the positive; ④The high expression of Ki-67 was worse than the low expression. ⑤ In molecular typing,luminal A<luminal B<over experssion of HER-2<triple negative. 2.The relationship between the abnormal expression of β-catenin with WHO,LN,M,pTNM,triple negative or not and Ki-67 was significant difference(P < 0.05) in IDC.①The less differentiation, the more abnormal expression of E-cad was; ② When the number of metastatic lymph nodes is ≥ 4, the film was significantly decreased. ③Distant metastasis was significantly higher than that in non membrane expression of metastasis has occurred; ④ The type of basal-like was much worse non basal-like;over expression of HER-2 showed a more prominent cytoplasmic metachromatic ⑤ Over expression of Ki-67 showed more intense capsule loss.3.The loss of E-cad membrane and the cytoplasm of the beta-catenin over expression,membrane of the β-catenin over expression was positively correlated.The loss of E-cad in membrane,DCIS-MI>IDC>DCIS>fibroadenoma. E-cad in membrane loss may occur early in invasive cancer has occurred. Loss of β-catenin of DCIS-MI > DCIS > fibroidadenoma. With the increase of β-catenin film invasive tumor cells loss is more obvious.5.To detect the expression of β-catenin beta may predict early metastasis of human breast cancer, As one of the human breast cancer prone to distant metastasis risk index.6.The combined detection of E-cad and β-catenin and the clinicopat-hologic features of breast cancer may improve the prediction of invasive breast cancer cells.7.The abnormal expression of β-catenin in cytoplasmic of IDC was unknown, needing for further research.