Effect Of TRAIL And Its Receptor In Systemic Lupus Erythematosus

Background Systemic lupus erythematosus(SLE) is a chronic autoimmune disorder of unknown etiology and its mechanism is complex that may involve multiple systems and organs.The characteristics of SLE included prolonged course, high recurrence rate, high mortality rate. Apoptosis / necrosis cell clearance failure led to a large number of autoimmune antigen cell components exposed to the cell surface is the immune response mechanism to expand with one of the potential endogenous. So how to reasonable regulating or blocking apoptosis pathway has become an important research focus to the treatment of SLE effectively.TRAIL and its receptor is a key factor in the regulation of apoptosis pathway. Although there has been some studies about TRAIL and its receptor in systemic lupus erythematosus pathologically and these studies usually concentrates on the experimental models and clinical detection of TRAIL-Rs and related factors. And the TRAIL receptor is seldom studied except the death receptor. Regulation of TRAIL and its receptor in the pathogenesis of SLE has the versatility in the formation mechanism, and highly complex, need further systematic study.Objective: To detect the peripheral mononuclear cells(PBMCs) in the expression of TRAIL and its five receptors in SLE patients,to provide the theoretical basis for further study on the occurrence, TRAIL and its five receptors in SLE developing action.Materials and methods: The PBMCs of 20 patients with SLE and 10 normal human peripheral venous blood, human lymphocyte separating soulutions PBMCs, extract the RNA after reverse transcription kit reversal in c DNA, by real-time fluorescent quantitative PCR method, detecting the expression level of SLE patients and normal human PBMCs TRAIL and its five receptors.β-actin was used as endogenous control, the relative expression levels of TRAIL and its five receptors in PBMCs of systemic lupus erythematosus patients were calculated by 2-ΔΔCT method, and analysis of correlation between five kinds of key and the molecular mechanism of the pathogenesis of TRAIL in systemic lupus erythematosus. Use SPSS17.0 statistical software for statistical analysis on the experimental data, the alpha = 0.05 as inspection standard.Results: 1. The TRAIL and five kinds of receptors in most patients with lupus erythematosus(SLE) and normal group of PBMCs were expressed.2.In the statistical analysis showed that the TRAIL-R1(P < 0.05) expression of higher than normal group, TRAIL and TRAIL-R2(P < 0.01) expression significantly higher than normal group, TRAIL-R3, the TRAIL-R4 expression change in the normal group, but no significant difference(P > 0.05),TRAIL-R5 express lower than normal group(P < 0.05).Conclusion:1.The TRAIL and five kinds of receptors expressed in most of SLE patients and normal control group at the same time.2.The expression of TRAIL-R2 significantly higher than other several gene expression, show the path of SLE patients with abnormal apoptosis induced by TRAIL and TRAIL-R2 mediated pathway may play an important role. 3. The TRAIL-R5 has protective effect on bone tissue, in this experiment, TRAIL-R5 lower than normal group, speculated that the bone metabolic abnormalities and SLE patients may have relations.