| Gastric cancer(GC) is a common malignant tumor, the mortality and morbidity in the country are significantly more than the global average, seriously endangering the public health. Currently treatment of a variety of GC, GC long-term survival but no significant improvement, because of its etiology and formation mechanism is not very clear. In recent years, we found that insulin-like growth factors(IGFs) in the body is important during mitosis, their proliferation and anti-apoptotic activity was found in a variety of tumors, currently found in breast cancer, colon cancer, ovarian cancer and other malignant tumor expression levels were significantly increased, and shown to promote cancer invasion and metastasis.In recent years, a series of studies have shown that: IGFs increased expression may promote the formation and development of malignant tumors, people with type 2 diabetes mellitus(T2DM) population, malignant tumors and blood circulation, IGFs expression were significantly higher than normal, while abnormal expression could promote tumorigenesis. This study was to detect simple GC in IGF-1, IGF-1R and IGFBP-2 in patients with carcinoma in patients with T2DM-GC expression, and compares it with the adjacent normal stomach tissue analysis in GC formation and development of T2 DM the possible role played in its intermediate links, then study the IGFs and GC invasion and metastasis, clinical stage between clinicopathological parameters such relations, on the one hand providing new biological indicators for determining the degree of malignancy and prognosis of GC; the other On the one hand provide new therapeutic targets for patients with T2DM-GC,thereby providing theoretical support optimal selection of this group for comprehensive treatment program.Objective:Observation of IGF-1, IGF-1R and IGFBP-2 in gastric cancer were simple, with T2DM-GC group and adjacent normal gastric tissues, the expression of each factor to observe and analyze the clinical and pathological features between relationship, to explore three factors in GC patients with T2 DM genesis and development of clinical significance, preliminary research T2 DM form relations with GC, GC expect to achieve early clinical findings, invasion and metastasis, and postoperative monitoring provides a new biological marker objects, and provide possible new targets and some theoretical support for the treatment of GC patients with T2 DM.Method:This topic using immunohistochemical methods IGF-1,IGF-1R, IGFBP-2,respectively purely GC(92 cases), normal gastric mucosa(27cases), T2DM-GC(46 cases) and normal stomach with T2 DM mucosa(19cases) tissue expression,and by studying the relationship between the factor and GC clinical parameters analyzed differential expression levels of three factors in different pathological features of the GC,their clinical significance,the factors related to the expression sex.Result:1 The expressions and the relationship with clinicopathological features of IGF-1,IGF-1R and IGFBP- 2 in pure GC group.IGF-1,IGF-1R and IGFBP-2 in pure GC positive group was significantly higher than in normal tissues(51.1% vs 18.5%,60.9% vs29.6%,66.3%vs 25.9%),are present statistics significance(P<0.05)(see Table 1,Table 3,Table 5).IGF-1 expression and infiltration of GC GC in simple, and lymph node metastasis and TNM staging correlated(P<0.05),but not with other clinicopathological features no correlation(P>0.05),which is deep infiltration, lymph node metastasis and TNM staging group later expressed higher rate than the control group, the difference was statistically significant(P<0.05); IGF-1R expression and infiltration of GC GC in the simple, primary tumor size, lymph node metastasis and there TNM staging correlated(P <0.05),and other clinicopathological features no correlation(P>0.05), its deep infiltration in primary tumor is large, with lymph node metastasis and TNM staging group later The expression was significantly higher than the control group, the difference was statistically significant(P<0.05); IGFBP-2expression and infiltration of GC GC in the simple, primary tumor size, distant metastasis, lymph node metastasis and TNM staging Related sex(P<0.05),but not with other clinicopathological features no correlation(P>0.05),which in a large primary tumor, infiltration depth,with distant metastasis,lymph node metas- tasis and TNM staging group later The expression was significantly higher than the control group, the difference was statistically significant(P<0.05)(see Table 2,Table 4,Table 6).2 The expression and the relationship with clinicopathological features of IGF-1, IGF-1R and IGFBP- 2 in GC with T2 DM group.IGF-1, IGF-1R and IGFBP-2 expression in the GC with T2 DM group was significantly higher than that of normal gastric mucosa associated with T2DM(71.7% vs 36.8%,80.4% vs31.6%, 76.1%vs 47.4%),statistically significant(P<0.05)(see Table 1, Table 3, Table 5).GC expression with infiltration of IGF-1 in T2 DM, lymph node metastasis and TNM staging correlated(P<0.05),while the other features clinical case no correlation(P>0.05),its infiltration in deeper with lymph node metastasis and TNM staging group later expression was significantly higher than the control group, the difference was statistically significant(P<0.05); the expression of IGF-1R and the GC with T2 DM primary tumor size, depth of invasion, lymph node metastasis and TNM staging correlated(P<0.05), while the other features clinical case no correlation(P>0.05), which in the primary tumor is large,deep infiltration,lymph node metastasis and TNM later Staging group expression was significantly higher than normal group(P<0.05), statistically significant differences(P<0.05); IGFBP-2 and GC with T2 DM of primary tumor size, depth of invasion, lymph node metastasis, distant Transfer Office and TNM staging correlated(P<0.05),while the other features clinical case no correlation(P>0.05),which in a large primary tumor, infiltration depth, lymph node metastasis and distant metastasis and better Night TNM staging group expression was significantly higher than normal group(P<0.05), statistically significant differences(P<0.05)(see Table 2, Table 4, Table 6).3 The influence of T2 DM to IGF-1,IGF-1R,IGFBP-2 expression in GC tissues.Expression of IGF-1 and IGF-1R in T2DM-GC group high(71.7% vs 51.1%, 80.4% vs60.9%) than the simple GC group,the differences were statistically significant(P<0.05), both the combined expression in normal gastric mucosa T2 DM was no significant difference(36.8% vs 18.5%,31.6% vs 29.6%),were not statistically significant(P>0.05);IGFBP-2 in T2DM-GC group higher expression of pure GC group, but they showed no statistical significance(76.1% vs 66.3%)(P>0.05); IGFBP-2 expression in gastric mucosa with T2 DM was also higher than in the normal mucosa group(47.4% vs 25.9%), both not statistically significant comparison(P>0.05)(see Table 1, Table 3,Table 5).4 The correlation about the expression of IGF-1,IGF-1R,and IGFBP-2 in GC and in GC with T2 DM group.Correlation analysis showed that: in pure GC(92 cases) in IGFBP-2 and IGF-1, IGF-1R expression showed a positive correlation(P<0.05,P<0.05, P<0.05); the merger of T2 DM GC(46 cases) and IGFBP-2 and IGF-1, IGF-1R expression also showed a positive correlation(P<0.05,P<0.05,P<0.05),the statistics of all GC(138 patients) in the combined analysis showed expression of IGF-1R IGFBP-2 also showed between IGF-1 and IGF-1R and IGF-1 positive correlation(P<0.05,P<0.05,P<0.05).Conclusions:1 IGF-1,IGF-1R,IGFBP-2 might have participated in the formation and development of GC, and the third is to promote the risk factors caught GC occurred.2 IGF-1,IGF-1R, IGFBP-2 promote GC invasion and metastasis, three plays synergy.3 GC on the cause of the occurrence of T2 DM promote discussion, this study shows:IGFs family may play a very important role. |
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