Effects Of Lutein On The Proliferation And Apoptosis Of Human Cervical Cancer Hela Cells

Background&objective:The 2009 Chinese cervical cancer incidence and mortality analysis report suggested that the incidence and mortality of cervical cancer are all on the rise.In recent yeas,cervical cancer has the trend of the group is getting younger.So far,the treatment of cervical cancer include surgical treatment,supplemented by radiotherapy and chemotherapy,but radiotherapy and chemotherapy side effects and drug resistance has become a major obstacle to cervical cancer treatment. Lutein is a carotenoid contained ionone ring,which can also play many biological functions in antioxidant,prevent cataracts,delay atherosclerosis and anti-tumor.At present,there has been no report of effects of lutein on the cervical cancer. The article aims to explore the effects of lutein on the proliferation and apoptosis of human cervical cancer HeLa cells.Methods:The human cervical cancer HeLa cells were cultivated in the incubator which contained 10% fetal bovine serum RPMI 1640 medium and placed in 37℃,5% CO2,95% air humidified.Using a random number table,those HeLa cells were randomly divided into six groups: control group,20 μmol/L lutein group,40 μmol/L lutein group,80 μmol/L lutein group,160μmol/L lutein group and 320 μmol/L lutein group,then added an equal volume of RPMI 1640 medium and RPMI1640 medium that contained 20 μmol/L,40 μmol/L,80 μmol/L,160 μmol/L,320 μmol/L final concentration of lutein, respectively.The HeLa cells proliferation inhibitory rates were determined by methyl thiazolyl tetrazolium(MTT) assay and the apoptosis rates of HeLa cells were determined by flow cytometry.Using statistical methods to analyze the differences of proliferation inhibitory rates and apoptosis rates of human HeLa cells that cultured the same time but in different concentrations of lutein and cultured in the same concentration of lutein but at different time points.Result:When HeLa cells cultured the same time(24 h or 48 h or 72 h),with lutein concentration increased by turns(20μmol/L,40μmol/L,80μmol/L,160μmol/L,320μmol/L,respectively),compared with the previous low concentration group,the proliferation inhibitory rates and apoptosis rates increased observably,all the differences mentioned above were of statistical criteria (P<0.01);When HeLa cells cultured in the same concentration(20μmol/L or 40μmol/L or 80μmol/L or 160μmol/L or 320μmol/L) of lutein,with the culture time extended (24h,48h,72 h,respectively),compared with the previous time point,the proliferation inhibitory rates and apoptosis rates were observably increased,all the differences mentioned above were of statistical criteria P<0.01).Conclusions:Lutein can inhibit the cell proliferation and induce the apoptosis of in vitro human HeLa cells.