| Coronary heart disease (CHD) poses one of the most serious threat to human health these days and has caused high mortality. Atherosclerosis is the pathological cause of it. The development of endothelial cell damage is closely related with CHD. As a Precursor cell of endothelial cells, EPCs (endothelial Progenitor cells, EPCs) plays a vital rolein tissue ischemia, in mobilizing the cells into blood to generate caPillaries and to repair microvascular endothelium in the course of vascular injury or damage, and in the process of occurrence and development of CHD.It has been proved that the decreasing in the number of EPCs and its dysfunction has close relationship with the incidence of CHD. This article will elaborateon the changes in the number of and the dysfunction of endothelial progenitor cells in peripheral blood of the patients.PurPose:To investigated the different altitudes between the changes of Lanzhou (elevation:1520m) and Xianyang (elevation: 386m) in coronary heart disease patient’s function of Peripheral blood Progenitor cell numbersMeans:Determination of coronary heart disease Patients and healthy peripheral blood EPCs surface marker CD45 antigen sPecificity of the two regions by flow cytometry, CD34, KDR expression rate of; function measured in Peripheral blood serum factor IL-8 (interleukin-factor by ELISA 8), VEGF (vascular endothelial growth factor), HCY (homocysteine), HIF-la (hypoxia inducible factor-la), SDF-la (stromal cell derived factor la); quantitative PCR in Peripheral blood mononuclear monocytes, CXCR2 mRNA, CXCE4 mRNA, CXCR7 mRNA expression;Results:Both the number of EPCs are relatively healthy PeoPle with coronary artery disease increased, and Lanzhou City and Xianyang City, the number of EPCs was no significant difference in CHD group, Xianyang City, compared with the healthy group increased the number of healthy group Lanzhou EPCs, there are significant differences (P<0.05). Two comparative analysis of C-reactive Protein derived CHD group than the control group were highly expressed, and altitude effects on CRP results were not significant.ELISA test results: ①IL-8:male CHD group compared Xianyang Lanzhou upregulated (341.4±209.7; 97.92±41.8), Xianyang City men with coronary heart disease group was comPared with the healthy grouP also raised (428±295.72; 97.92±41.8) were different (P<0.05). ②VEGF: Xianyang City CHD groupcompared with the healthy group were upregulated (712±319.27; 2.43±1.68) (P<0.05). Lanzhou male CHD group compared Xianyang upregulated (1.73±1.35; 12.2±3.3) (P<0.05). ③HCY: Lanzhou City and Xianyang City health groups are more highly expressed in CHD group and women have differences in Lanzhou (P <0.05) and Xianyang City men; men CHD group compared Xianyang Lanzhou upregulated (7.99±3.07; 18.7±12.67) (P<0.05). ④SDF-la: Lanzhou City and Xianyang City men’s health groups were highly expressed in the CAD group (3.9± 1.7; 10.03±5.18 and 2.20±0.92; 7.08±4.22) (P<0.05); male CHD group compared Xianyang Lanzhou City upregulated (4.08±3.8; 2.2±0.92) (P<0.05). ⑤HIF-1a: Xianyang City, coronary heart disease group was compared with the healthy group raised significant difference (2.68±1.84; 1.27±0.79) (P<0.05). The Lanzhou City and Xianyang City, coronary heart disease group and healthy comparison group were up-regulated expression, and the difference was significant. Quantitative PCR results: Lanzhou City, Xianyang City CXCR2 expression representing a significant difference (P<0.05).Conclusion:EPCs in patients with coronary heart disease than in healthy people the number decreased. Low altitudes higher altitudes healthy people to increase the number. IL-8, VEGF, HCY, HIF-1a, SDF-1a, CXCR2 factor under study conditions at different altitudes and gender EPCs function was meaningful and broad Prospects. |
