| This dissertation is carried on the chemical and pharmacological activities study on Momordica cochinchinensis(Lour.) Spreng. and Camellia fengchengensis Liang et Zhong. Twenty-five compounds including two new ones were isolated from the two plants, by using varied chromatographic methods and separation techniques. These compounds refered to oleanane-type triterpene and flavan-3-ol. Their structures were elucidated by the detailed spectroscopic analysis, including 1D, 2D NMR, IR and MS techniques. Most of compounds were tested for their cytotoxic activity and glucose uptake activity. Some of them exhibited the weak activity. Moreover, the volatile constituents of tea samples produced from Lincang(Yunnan province) were obtained by hydrodistillation and analyzed by GC-MS analysis. Furthermore, the identification, chemical and pharmacological activities study on the genus Momordica were reviewed.The detailed contents are shown as following:Chapter 1. The Chemical constituent and pharmacological activities study of Momordica cochinchinensis(Lour.) Spreng. The phytochemical and pharmacological activities studies on Momodica charantia were abundant, while M. cochinchinnensis are relatively less. M. cochinchinensis, a traditional Chinese medicine, is mainly found in southern parts of China, also distributed in India. Two new oleanane-type triterpenoid saponins(1 and 2) were isolated from the methanolyzed total saponins of the seeds of Momordica cochinchinensis(Lour.) Spreng, together with 18 known compounds(3–20). Their structures were elucidated on the basis of detailed spectroscopic analyses, including MS, 1D, and 2D NMR, as well as methanolysis and LC-MS analysis. All the isolates were tested for their cytotoxic activities against five human cancer cell lines(HL-60, SMMC-7721, PANC-1, A-549, and SW-480) and the glucose uptake activity. The known compound 6 exhibited weak toxicity effects against HL-60 with IC50 values of 18.13 μM, while 10 showed weak cytotoxicity against SMMC-7721 and A-549 cell lines, with IC50 values of 34.43 and 32.80 μM, respectively. In addition, the new compound 2 showed weak glucose uptake activity with the glucose consumption value of 0.292 μM at 10 u M concentrationChapter 2. The Chemical constituent study of Camellia. fengchengensis Liang et Zhong.Camellia. fengchengensis Liang et Zhong., belong to the members of Camellia sect. Thea, is an endemic species distributed in Guangxi Province. Detailed chemical investigation on the green tea produced from the leaves of C. fengchengensis lead to the identification of 5 compounds, including procyanidin B3, procyanidin B2, epicatechin, catechin-7-O-β-D-glucopyranoside, catechin.Chaper 3. The aromatic constituents of tea samples produced from LincangLincang has a long history of black tea, which is one of the birthplaces of tea. In this study, seven representative camellia species in lincang were picked out, which were obtained by hydrodistillation and analyzed by GC-MS analysis. Sixty-nine compounds of the essential oil were identified.Chapter 4. Advances of chemical constituents and pharmacological activities of the genus MomordicaThis dissertation reviewed the chemical and pharmacological activities study on the genus Momordica. The genus Momordica(Cucurbitaceae), comprosing of about 80 species, is mainly distributed in Africa. Four species of which can be found in the south and southwest of China. Phytochemical studies demonstrated the presence of tetracyclic triterpenoids and its saponins, oleanane and ursane type pentacyclic triterpenoids and their saponins, steroids, organic acids, and amino acids in the plants of the genus Momordica. Pharmacological activities, such as hyperglycemic, anticancer, antibacterial, antiviral, immunoregulation, antiallergic, analgesic and antihypertension, have been reported. In this review, summarized the advances of chemical constituents and pharmacological activities of Momordica species since 1980, for further development and utilization of the resources of genus Momordica. |
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