| Objective This study through the application of contrast-enhanced ultrasound quantitative analysis ofischemia reperfusion injury at different time points before and after the rabbit liver contrast agentperfusion, to evaluate the value of application of this technology in the observation of the livermicrocirculation in.Method New Zealand 45 healthy adult rabbits, male or female, weighing 2.5 to 3.5kg,3-4 months of age, provided by the Experimental Animal Center of Guangxi Medical University, portal vein occlusion method to establish the use of rabbit liver ischemia-reperfusion model.45 healthy adult rabbits were randomly divided into sham operation group A, Pringle group, group B, Pringle partial hepatectomy group, Group C, respectively, before blocking after reperfusion Oh, 1h,6h,24h 5 CEUS examination time point line, offline rendering time-intensity curve (time-intensity curves, TIC), were estimated maximum peak intensity (maximum intensity, IMAX), the rise time (rising time, RT), peak time (time to peak, TTP), mean transit time (mean transit time, mTT) four TIC quantitative parameters. And at the same time CEUS points since ear vein blood, liver tissue, serum aspartate aminotransferase (Aspartate aminotransferase, AST) were detected, alanine aminotransferase (Alanine aminotransferase, ALT) levels and prepare biopsy, staining and histopathological observation.Results The results of quantitative ultrasound contrast analysis between groups group B, group C and group A the same time with the same parameters were significantly (p<0.05), group B, group C showed no significant difference (p> 0.05). A comparison of two groups within groups at different time points with the same parameters showed no significant difference (p> 0.05), group B, group C at different time points comparing with the same parameters, the parameters RT, TTP before reperfusion increased at all time points compared with blocking reperfusion 1h,6h,24h on the rise, and the difference was statistically significant (p<0.05), reperfusion Oh, 1h difference was not statistically significant (p> 0.05), parameter IMAX, mTT reperfusion 6h,24h and resistance before breaking the difference was statistically significant (p <0.05), IMAX was gradually, mTT was gradually increasing trend. Laboratory tests between the two groups group B, group C and group A the same time points serum ALT, AST levels were significantly (p<0.05), group B, group C showed no statistical significance (p> 0.05) within the two groups at different time points in group A comparison of serum ALT, AST levels had no significant difference (p> 0.05), group B reperfusion time points than before to block the increase, the difference was statistically significant (p<0.05), reperfusion time points serum ALT, AST level was gradually increased, and the difference was statistically significant (p<0.05). Pathology, Group B, C group showed Oh reperfusion edema liver cells, liver sinusoidal space narrowing, a large number of red blood cell aggregation in sinusoids and central veins (central vein, CV) within the lumen obstruction, micro-thrombosis; with reperfusion time the extension of reperfusion 1h can see red blood cell aggregation in hepatic sinusoids and periportal, blocking the lumen, resulting in microcirculation, periportal small amount of neutrophils; 6h after reperfusion edema was seen hepatocyte ballooning degeneration, and see a wide range of neutrophil infiltration.24h reperfusion liver cell atrophy, necrosis, sinusoid collapse.Conclusion Contrast enhanced ultrasound can accurate assessment of the liver microcirculation of liver ischemia reperfusion. |
